Extracellular Potassium and Astrocytic Kir4.1 Modulate Interneuron Activity in the Cerebral Cortex

细胞外钾和星形胶质细胞 Kir4.1 调节大脑皮层的中间神经元活动

• 批准号: 10784058
• 负责人: Moritz Armbruster
• 金额: $ 8.79万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-05-15 至 2027-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10784058
• 关键词: Action Potentials; Affect; Area; Astrocytes; Automobile Driving; Award; Biochemical; Biological Assay; Biology; Brain; Buffers; Cerebral cortex; Characteristics; Data; Data Analyses; Dependence; Development; Electrophysiology (science); Epilepsy; Epileptogenesis; First Generation College Students; Frequencies; Funding; Future; Hyperactivity; Image; In Vitro; Interneurons; Investigation; Latina; Measures; Mediating; Mentors; Mentorship; Modeling; Mutation; National Research Service Awards; Nervous System; Neurons; Neurosciences; Neurosciences Research; Neurotransmitters; Output; Parvalbumins; Pathologic; Pathway interactions; Physiological; Potassium; Qualifying; Research; Research Personnel; Role; Runaway; Seizures; Shapes; Signal Transduction; Site; Sleep Wake Cycle; Source; Statistical Data Interpretation; Structure; Synapses; Testing; Time; Training; Underrepresented Populations; Universities; Work; disadvantaged background; doctoral student; economic disparity; experience; extracellular; fluorescence imaging; gamma-Aminobutyric Acid; hippocampal pyramidal neuron; imaging approach; imaging modality; in vitro Model; insight; interest; inward rectifier potassium channel; live cell imaging; member; nervous system disorder; neuronal excitability; neurotransmission; neurotransmitter release; novel; overexpression; pre-doctoral; presynaptic; research faculty; spreading depression; synaptic function; tool; uptake; voltage

PROJECT SUMMARY This application is for a diversity supplement to the R01 NS127819-01 “EXTRACELLULAR POTASSIUM AND ASTROCYTIC KIR4.1 MODULATE INTERNEURON ACTIVTY IN THE CEREBRAL CORTEX” of Dr. Moritz Armbruster at Tufts University. The candidate is Reyna Gariepy, a pre-doctoral student in the lab of Dr. Armbruster. Reyna will take tools currently in use in the lab and use them to pursue her interests to investigate how GABA dynamics are shaped by astrocytes. Reyna qualifies as a diversity candidate as a Latina, first generation college student, and her economically disadvantaged background. Therefore, she is a member of multiple underrepresented groups. This supplement will fund Reyna for 3 years of her graduate thesis work. Funding for Y3 will be contingent on submission of an NRSA application during Y2. During this time Reyna will receive training in cellular neuroscience, glial biology, epilepsy and more. She will gain expertise in biochemical assays, astrocytes and neuronal electrophysiology, iGABASnFR imaging, and data and statistical analysis. This training will guide her towards an NRSA application and future as a leader in neuroscience research. Ms. Gariepy is devoted to academic research with a focus on epileptogenesis. Dr. Armbruster, is a junior research faculty in the Neuroscience Department at Tufts University who has extensive experience in astrocyte-neuron interactions and utilizing live-cell imaging approaches. To increase Reyna’s opportunities for development, we have included Dr. Chris Dulla as a senior mentor. Dr. Dulla has a great track record of mentorship, including the 2018 Landis Award for Outstanding Mentorship. The project laid out here will give Ms. Gariepy excellent and rigorous training in GABA neurotransmission, neuron/glial interactions, and cutting- edge imaging modalities. Dr. Armbruster, and Ms. Gariepy have developed a structured, rigorous, and customized mentorship and training plan. The funding offered through this supplement will give Reyna a clear path toward an exciting future as an up and coming researcher in the field of epilepsy and GABA neurotransmission. The activities laid out and the enrichment opportunities will be critical to Ms. Gariepy’s development and future as a successful independent investigator.

项目摘要 本申请是对R 01 NS 127819 -01“细胞外钾和 星形胶质细胞KIR 4.1调节大脑皮质中的中间神经元活性”莫里茨博士 塔夫茨大学的Armbruster。候选人是Reyna Garibe，博士生在博士的实验室。 阿姆布鲁斯特雷纳将利用实验室目前使用的工具并利用它们来追求她的兴趣进行调查 GABA动力学是如何被星形胶质细胞塑造的蕾娜有资格作为一个拉丁裔的多样性候选人，首先， 一代大学生，和她的经济弱势背景。因此，她是 多个代表性不足的群体。这个补充将资助雷纳3年的研究生论文工作。 Y3的资金将取决于在Y2期间提交NRSA申请。在此期间，Reyna将 接受细胞神经科学、神经胶质生物学、癫痫等方面的培训。她将获得以下方面的专业知识 生物化学测定、星形胶质细胞和神经元电生理学、iGABASnFR成像，以及数据和统计分析。 分析.这项培训将引导她走向NRSA应用程序和未来作为神经科学的领导者 research. Garibe女士致力于学术研究，重点是癫痫发生。Armbruster博士是一位 塔夫茨大学神经科学系的初级研究教师，他在以下方面拥有丰富的经验： 星形胶质细胞-神经元相互作用和利用活细胞成像方法。为了增加蕾娜 在开发过程中，我们邀请了Chris Dulla博士作为高级导师。Dulla博士有着良好的记录， 导师，包括2018年兰迪斯杰出导师奖。该项目将在这里提供 女士Garibe在GABA神经传递、神经元/神经胶质相互作用和切割方面接受了出色而严格的培训- 边缘成像模式。Armbruster博士和Garibrant女士已经制定了一个结构化，严谨， 定制的指导和培训计划。通过这一补充提供的资金将给雷纳一个明确的 通往一个令人兴奋的未来的道路，作为一个在癫痫和GABA领域的新兴研究人员 神经传递所安排的活动和丰富的机会对加里皮女士来说至关重要 作为一个成功的独立调查员的发展和未来。