Extracellular Potassium and Astrocytic Kir4.1 Modulate Interneuron Activity in the Cerebral Cortex

细胞外钾和星形胶质细胞 Kir4.1 调节大脑皮层的中间神经元活动

• 批准号: 10784058
• 负责人: Moritz Armbruster
• 金额: $ 8.79万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-05-15 至 2027-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10784058
• 关键词: Action Potentials; Affect; Area; Astrocytes; Automobile Driving; Award; Biochemical; Biological Assay; Biology; Brain; Buffers; Cerebral cortex; Characteristics; Data; Data Analyses; Dependence; Development; Electrophysiology (science); Epilepsy; Epileptogenesis; First Generation College Students; Frequencies; Funding; Future; Hyperactivity; Image; In Vitro; Interneurons; Investigation; Latina; Measures; Mediating; Mentors; Mentorship; Modeling; Mutation; National Research Service Awards; Nervous System; Neurons; Neurosciences; Neurosciences Research; Neurotransmitters; Output; Parvalbumins; Pathologic; Pathway interactions; Physiological; Potassium; Qualifying; Research; Research Personnel; Role; Runaway; Seizures; Shapes; Signal Transduction; Site; Sleep Wake Cycle; Source; Statistical Data Interpretation; Structure; Synapses; Testing; Time; Training; Underrepresented Populations; Universities; Work; disadvantaged background; doctoral student; economic disparity; experience; extracellular; fluorescence imaging; gamma-Aminobutyric Acid; hippocampal pyramidal neuron; imaging approach; imaging modality; in vitro Model; insight; interest; inward rectifier potassium channel; live cell imaging; member; nervous system disorder; neuronal excitability; neurotransmission; neurotransmitter release; novel; overexpression; pre-doctoral; presynaptic; research faculty; spreading depression; synaptic function; tool; uptake; voltage

PROJECT SUMMARY This application is for a diversity supplement to the R01 NS127819-01 “EXTRACELLULAR POTASSIUM AND ASTROCYTIC KIR4.1 MODULATE INTERNEURON ACTIVTY IN THE CEREBRAL CORTEX” of Dr. Moritz Armbruster at Tufts University. The candidate is Reyna Gariepy, a pre-doctoral student in the lab of Dr. Armbruster. Reyna will take tools currently in use in the lab and use them to pursue her interests to investigate how GABA dynamics are shaped by astrocytes. Reyna qualifies as a diversity candidate as a Latina, first generation college student, and her economically disadvantaged background. Therefore, she is a member of multiple underrepresented groups. This supplement will fund Reyna for 3 years of her graduate thesis work. Funding for Y3 will be contingent on submission of an NRSA application during Y2. During this time Reyna will receive training in cellular neuroscience, glial biology, epilepsy and more. She will gain expertise in biochemical assays, astrocytes and neuronal electrophysiology, iGABASnFR imaging, and data and statistical analysis. This training will guide her towards an NRSA application and future as a leader in neuroscience research. Ms. Gariepy is devoted to academic research with a focus on epileptogenesis. Dr. Armbruster, is a junior research faculty in the Neuroscience Department at Tufts University who has extensive experience in astrocyte-neuron interactions and utilizing live-cell imaging approaches. To increase Reyna’s opportunities for development, we have included Dr. Chris Dulla as a senior mentor. Dr. Dulla has a great track record of mentorship, including the 2018 Landis Award for Outstanding Mentorship. The project laid out here will give Ms. Gariepy excellent and rigorous training in GABA neurotransmission, neuron/glial interactions, and cutting- edge imaging modalities. Dr. Armbruster, and Ms. Gariepy have developed a structured, rigorous, and customized mentorship and training plan. The funding offered through this supplement will give Reyna a clear path toward an exciting future as an up and coming researcher in the field of epilepsy and GABA neurotransmission. The activities laid out and the enrichment opportunities will be critical to Ms. Gariepy’s development and future as a successful independent investigator.

项目摘要 该应用是为R01 NS127819-01的多样性补充 星形胶质细胞Kir4.1在莫里茨博士的大脑皮层中调节中间神经元活动 塔夫茨大学的臂章。候选人是雷纳·加里比（Reyna Gariepy），他是博士实验室的博士前学生。 臂章。雷纳将采用当前在实验室中使用的工具，并利用它们来追求她的兴趣来调查 GABA动力学如何由星形胶质细胞塑造。雷纳（Reyna）成为拉丁裔的多样性候选人，首先 一代大学生和她在经济上处于弱势背景。因此，她是 多个代表性不足的组。这种补充剂将为雷纳提供3年的毕业论文工作。 Y3的资金将取决于在Y2期间提交NRSA申请。在此期间，雷纳将 接受细胞神经科学，神经胶质生物学，癫痫等的培训。她将获得专业知识 生化测定，星形胶质细胞和神经元电生理学，IGABASNFR成像以及数据和统计 分析。这项培训将指导她作为神经科学领导者的NRSA应用和未来 研究。 Gariepy女士专注于癫痫发生。 Armbruster博士是 塔夫茨大学神经科学系的初级研究学院拥有丰富的经验 星形胶质细胞神经元相互作用并使用活细胞成像方法。增加雷纳的机会 发展，我们将克里斯·杜拉（Chris Dulla）博士包括在内。杜拉博士的记录很棒 Mentalship，包括2018年Landis杰出指导奖。这里布置的项目将给予 Gariepy女士在GABA神经传递，神经元/神经胶质相互作用和剪切方面进行了精美而严格的培训 边缘成像方式。 Armbruster博士和Gariepy女士已经开发了一个结构化，严格的 定制的精神制和培训计划。通过这种补充提供的资金将使雷纳明确 作为癫痫和GABA领域的新兴研究员，通往令人兴奋的未来的道路 神经传递。进行的活动和丰富的机会对Gariepy女士的 作为成功的独立研究者的发展和未来。