Autoimmunity Lymphocyte Repertoire Core (ALRC)

这一核心将为芝加哥大学自身免疫卓越中心(UCACE)提供服务 研究人员确定B淋巴细胞在自身免疫病理中的作用。有两种最先进的 将允许中心调查人员仔细检查自身抗体介导的病理的技术。 1.用重组单抗技术分析B淋巴细胞的特异性。 2.免疫球蛋白轻链可变基因芯片 最后，魏格特博士和威尔逊博士将为免疫球蛋白的研究提供协助和技术支持 采用单细胞聚合酶链式反应、克隆和测序。这种方法将增加轻链的使用。 基因芯片和人类单抗分析。 仔细研究人类B细胞介导的病理学的独特方法将为 中心帮助我们了解狼疮和重症肌无力。

This core will provide services to University of Chicago Autoimmunity Center of Excellence (UCACE) investigators to characterize the role of B lymphocytes in autoimmune pathology. There are two state-of-the-art technologies that will allow the center investigators to scrutinize autoantibody-mediated pathology. 1. Analysis of B lymphocyte specificity using recombinant monoclonal (mAb) technology. 2. Immunoglobulin light chain variable gene microarrays Finally, Drs. Weigert and Wilson will assist and provide technical support for studies of the immunoglobulin repertoire using single-cell PCR, cloning and sequencing. This approach will augment the use of light chain microarrays and human mAb analysis. The unique methodologies for scrutinizing human B cell mediated pathology will provide powerful tools to the center to facilitate our understanding of lupus and myasthenia gravis.