Autoimmunity Lymphocyte Repertoire Core (ALRC)

自身免疫淋巴细胞库核心 (ALRC)

• 批准号: 7688953
• 负责人: Patrick Christopher Wilson
• 金额: $ 21.88万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-10 至 2014-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7688953
• 关键词: Antibodies; Antibody Formation; Antibody Specificity; Antigens; Arts; Autoantibodies; Autoimmune Diseases; Autoimmune Process; Autoimmunity; B cell repertoire; B-Lymphocytes; Binding; Biology; Cell Count; Cells; Chicago; Clinical Trials; Clonality; Cloning; Data; Evaluation; Flow Cytometry; Generalized Myasthenia Gravis; Generations; Genes; Genetic Materials; Human; Hybridomas; Immunity; Immunoglobulins; Invaded; Kidney; Laboratories; Lead; Learning; Light; Light-Chain Immunoglobulins; Lupus; Lymphocyte; Measures; Mediating; Methodology; Microarray Analysis; Monoclonal Antibodies; Mus; Myasthenia Gravis; Neuromyelitis Optica; Oncogenic Viruses; Paper; Pathology; Patients; Phenotype; Pilot Projects; Population; Process; Publishing; Recombinant Antibody; Recombinants; Research Personnel; Research Project Grants; Resistance; Role; Services; Somatic Mutation; Sorting - Cell Movement; Source; Specificity; System; Systemic Lupus Erythematosus; Technology; Testing; Universities; Virus Diseases; aquaporin 4; authority; autoreactive B cell; autoreactivity; design; insight; member; neoplastic; new technology; peripheral blood; receptor; rituximab; tool

This core will provide services to University of Chicago Autoimmunity Center of Excellence (UCACE) investigators to characterize the role of B lymphocytes in autoimmune pathology. There are two state-of-the-art technologies that will allow the center investigators to scrutinize autoantibody-mediated pathology. 1. Analysis of B lymphocyte specificity using recombinant monoclonal (mAb) technology. 2. Immunoglobulin light chain variable gene microarrays Finally, Drs. Weigert and Wilson will assist and provide technical support for studies of the immunoglobulin repertoire using single-cell PCR, cloning and sequencing. This approach will augment the use of light chain microarrays and human mAb analysis. The unique methodologies for scrutinizing human B cell mediated pathology will provide powerful tools to the center to facilitate our understanding of lupus and myasthenia gravis.

该核心将为芝加哥大学自身免疫卓越中心（UCACE）提供服务 研究者描述B淋巴细胞在自身免疫病理学中的作用。有两个最先进的 这些技术将允许中心研究人员仔细检查自身抗体介导的病理学。 1.利用重组单克隆抗体技术分析B淋巴细胞特异性。 2.免疫球蛋白轻链可变区基因芯片 最后，Weigert和Wilson博士将协助并为免疫球蛋白的研究提供技术支持。 使用单细胞PCR、克隆和测序来鉴定所有库。这种方法将增加轻链的使用， 微阵列和人mAb分析。 用于仔细检查人B细胞介导的病理学的独特方法学将为研究人B细胞介导的病理学提供有力的工具。 中心，以促进我们对狼疮和重症肌无力的了解。