Monoclonal Antibody and Biosensor Core

SERCEB的计划对单抗有直接和预期的需求 用于研究发病机制、免疫反应、疫苗开发、 治疗和诊断学。这些试剂和生物识别元件将对 建议的研究项目取得成功。单抗的性质和来源 所需的将取决于研究计划的目标，范围从小鼠单克隆 人或人源化小鼠抗体的免疫组织化学和检测 用于治疗学的发展。SERCEB的机构内有广泛的专业知识 在单抗的开发、测试和使用中。单抗研发方面的专业知识包括 人、小鼠、人源化小鼠、恒河猴和噬菌体展示人和小鼠的腹肌和 ScFv.最后，抗体将是可交付的，可能立即应用于 治疗和诊断学。建议的核心C是一个分布式核心，它使用已建立的 在几个机构设立实验室，以提供所需的每一项服务。核心将提供所有 SERCEB所需的生物识别元素，并将把它们开发成新的检测方法和 生物传感器。 核心有四个具体目标。 目的1.制备抗痘病毒中和靶和炭疽保护作用的人源单抗 抗原。 目的2.为SERCEB研究项目制备高质量的单抗试剂。 目标3.利用新型单抗开发用于诊断或检测选定试剂的生物传感器 在SERCEB中提供 目标4.在SERCEB内为单抗开发一个数据库和储存库，可以 在各机构之间共享，以促进其他科学项目。

The programs of the SERCEB have immediate and projected requirements for monoclonal antibodies (mAbs) for studies of pathogenesis, immune response, vaccine development, therapeutics and diagnostics. These reagents and bio-recognition elements will be vital to the success of proposed research projects. The nature and source of the monoclonal antibodies required will depend on the objective of the research program, ranging from murine monoclonal antibodies for immunohistochemistry and detection to human or humanized mouse antibodies for development of therapeutics. Broad expertise exists within the institutions of the SERCEB in mAb development, testing and use. The spectrum of expertise in mAb development includes human, mouse, humanized mouse, rhesus, and phage display human and mouse F abs and ScFv. Finally, the antibodies will be deliverables that may have immediate application in therapeutics and diagnostics. The proposed Core C is a distributed core that uses established labs at several institutions to provide each of the needed services. The Core will provide all of the biorecognition elements needed in the SERCEB and will develop them into new assays and biosensors. The core has four Specific Aims. Aim 1. Generate human mAbs to poxvirus neutralization targets and anthrax protective antigen. Aim 2. Generate high-quality mAb reagents for SERCEB research programs. Aim 3. Develop biosensors for diagnostics or detection of select agents using novel mAbs available in the SERCEB Aim 4. To develop a database and repository for mAbs within the SERCEB that can be shared across institutions, to facilitate the other scientific programs.