Monoclonal Antibody and Biosensor Core

单克隆抗体和生物传感器核心

• 批准号: 7656437
• 负责人: James E Crowe
• 金额: $ 38.6万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-15 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7656437
• 关键词: Aloral; Antibodies; Antigens; Bacillus anthracis spore; Bacterial Adhesins; Binding; Biological Assay; Biosensor; Cell Line; Complement; Cowpox; Cytokine Gene; Databases; Detection; Development; Diagnosis; Diagnostic; Elements; Human; IRAK3 gene; Immune; Immune response; Immunization; Immunohistochemistry; Institution; Interleukin-10; Interleukin-4; Macaca mulatta; Monoclonal Antibodies; Mus; Nature; Pathogenesis; Phage Display; Poxviridae; Production; Protein Fragment; Proteins; Range; Rattus; Reagent; Receptor Gene; Recombinants; Replicon; Research; Research Project Grants; Retroviridae; SECTM1 gene; Services; Source; System; Testing; Therapeutic; Vaccinia; Work; Yersinia pestis; anthrax protective factor; concept; interleukin-18 receptor; novel; programs; repository; sensor; success; vaccine development; vector

The programs of the SERCEB have immediate and projected requirements for monoclonal antibodies (mAbs) for studies of pathogenesis, immune response, vaccine development, therapeutics and diagnostics. These reagents and bio-recognition elements will be vital to the success of proposed research projects. The nature and source of the monoclonal antibodies required will depend on the objective of the research program, ranging from murine monoclonal antibodies for immunohistochemistry and detection to human or humanized mouse antibodies for development of therapeutics. Broad expertise exists within the institutions of the SERCEB in mAb development, testing and use. The spectrum of expertise in mAb development includes human, mouse, humanized mouse, rhesus, and phage display human and mouse F abs and ScFv. Finally, the antibodies will be deliverables that may have immediate application in therapeutics and diagnostics. The proposed Core C is a distributed core that uses established labs at several institutions to provide each of the needed services. The Core will provide all of the biorecognition elements needed in the SERCEB and will develop them into new assays and biosensors. The core has four Specific Aims. Aim 1. Generate human mAbs to poxvirus neutralization targets and anthrax protective antigen. Aim 2. Generate high-quality mAb reagents for SERCEB research programs. Aim 3. Develop biosensors for diagnostics or detection of select agents using novel mAbs available in the SERCEB Aim 4. To develop a database and repository for mAbs within the SERCEB that can be shared across institutions, to facilitate the other scientific programs.

SERCEB的计划对单克隆抗体有直接和预计的需求。 用于研究发病机理、免疫应答、疫苗开发、 治疗和诊断。这些试剂和生物识别元件将对生物识别至关重要。 成功的研究项目。单克隆抗体的性质和来源 所需的将取决于研究计划的目标，从鼠单克隆抗体， 用于免疫组织化学和检测人或人源化小鼠抗体的抗体 用于开发治疗方法。在SERCEB的机构内有广泛的专门知识 mAb的开发、测试和使用。mAb开发的专业知识范围包括 人、小鼠、人源化小鼠、恒河猴和噬菌体展示人和小鼠F abs， 单链抗体最后，抗体将是可交付的，可以立即应用于 治疗和诊断。拟议的核心C是一个分布式核心， 在几个机构的实验室，以提供每一个需要的服务。核心将提供所有 SERCEB中所需的生物识别元素，并将其开发成新的检测方法， 生物传感器 核心有四个具体目标。 目标1.产生针对痘病毒中和靶标和炭疽保护性的人mAb 抗原的 目标2.为SERCEB研究项目生成高质量的mAb试剂。 目标3.使用新型mAb开发用于诊断或检测选定试剂的生物传感器 在SERCEB中可用 目标4。为了在SERCEB内开发一个mAb数据库和储存库， 跨机构共享，以促进其他科学计划。