Detection of Anthrax Infection by Magnetic Reporting of Molecular Sensors

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Tufts University Medical School Dr. Edward Goldberg is designing molecular sensors that will use magnetic reporting to detect anthrax infection. The long-term goal is to develop a hand-held diagnostic device that will use a non-destructive detection method to identify anthrax in patient samples. They are using bacteriophage tail fiber protein to determine the magnetic susceptibility of protein-nanoparticle conjugates for use as detection biosensors. He requested the production of E. coli cell pellets infected with a mutant form of the long tail fiber of bacteriophage T4. ¿. co// cultures were grown and infected with bacteriophage. Because we use fermentors with a programmable control system we were able to provide the fast growing culture with sufficient oxygen supply, a problem Dr. Goldberg had encountered in his previous efforts in his own laboratory. We completed one fermentation run that generated 180 g of cell pellet which was further processed in Dr. Goldberg's laboratory.