Functional Genomic Studies of Neuronal Differentiation
神经元分化的功能基因组研究
基本信息
- 批准号:7560357
- 负责人:
- 金额:$ 56.66万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2006
- 资助国家:美国
- 起止时间:2006-01-15 至 2010-12-31
- 项目状态:已结题
- 来源:
- 关键词:ASCL1 geneAlzheimer&aposs DiseaseBinding SitesBioinformaticsBiological ModelsBiological ProcessCell Differentiation processCellsCis-Acting SequenceCollectionComplexConserved SequenceCuesDevelopmentDown-RegulationElementsEmbryoEventEvolutionFoundationsFutureGene ExpressionGenesGenetic ProgrammingGenieGenomicsIn Situ HybridizationLaboratoriesLigandsMalignant Epithelial CellMediatingMethodologyMethodsNervous system structureNeurodegenerative DisordersNeuronal DifferentiationNeuronsParkinson DiseasePlasmidsProcessProtein KinaseProteinsRNA InterferenceReagentRegulationRegulatory PathwayReporterResearch PersonnelRoleSignal PathwaySignal Transduction PathwaySmall Interfering RNASpecific qualifier valueStem cellsTestingTherapeuticTranscriptional ActivationTranscriptional RegulationTransfectionTretinoinbasebiological systemsextracellularfunctional genomicsin vivomammalian genomemouse genomenerve stem cellnervous system developmentneuron developmentprogenitorprogramsresearch studyresponsetranscription factorvector
项目摘要
The precise differentiation of neurons within the vertebrate nervous system is specified by a complex
genetic program of transcription factor interactions, as well as modulation of these interactions by
environmental cues. Although the roles of many specific transcription factors and signaling pathways have
been characterized in neuronal differentiation, our understanding of the detailed mechanisms involved is still
far from complete. This proposal employs genomic sequence information of several mammalian genomes,
the fundamental observation that vertebrate nervous system development is highly conserved throughout
evolution, and recent developments in functional genomics to propose a high-throughput functional genomic
analysis of neuronal differentiation. The hypothesis to be tested in this proposal is that evolutionary
conserved sequences surrounding genes induced during neuronal differentiation are important to the
orchestration of neuronal induction. In the first specific aim,P19 embyronic carcinoma cells will be induced
to undergo neuronal differentiation and microarray hybridization studies will be carried out to identify genes
that are transcriptionally regulated. In the second specific aim, evolutionarily conserved non-genie
sequences (CNGs) of the mouse genome, that have recently been proposed to represent clusters of cis-
regulatory sequences, will be analyzed for transcriptional regulation using a high-throughput microarray
transfection method in P19 cells. In the third specific aim, the role of protein kinases and transcription
factors in the regulation of these cis- acting sequences will be characterized. The end result of these studies
will be a detailed understanding of important cis-regulatory sequences of neuronally induced genes and the
role of specific signal transduction pathways in the modulation of their transcriptional activation. The results
of these studies will be important for future therapeutic approaches to treatment of neurodegenerative
diseases including Alzheimer's and Parkinson's diseases.
脊椎动物神经系统中神经元的精确分化由一个复合体来指定
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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MICHAEL D UHLER其他文献
MICHAEL D UHLER的其他文献
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{{ truncateString('MICHAEL D UHLER', 18)}}的其他基金
Functional Genomic Studies of Neuronal Differentiation
神经元分化的功能基因组研究
- 批准号:
7342455 - 财政年份:2006
- 资助金额:
$ 56.66万 - 项目类别:
Functional Genomic Studies of Neuronal Differentiation
神经元分化的功能基因组研究
- 批准号:
7048771 - 财政年份:2006
- 资助金额:
$ 56.66万 - 项目类别:
Functional Genomic Studies of Neuronal Differentiation
神经元分化的功能基因组研究
- 批准号:
7167717 - 财政年份:2006
- 资助金额:
$ 56.66万 - 项目类别:
Postgenomic approaches to diabetic complications
糖尿病并发症的后基因组学方法
- 批准号:
6666784 - 财政年份:2002
- 资助金额:
$ 56.66万 - 项目类别:
Postgenomic approaches to diabetic complications
糖尿病并发症的后基因组学方法
- 批准号:
6574953 - 财政年份:2002
- 资助金额:
$ 56.66万 - 项目类别: