HIV-envelope-specific CD4+ T-cell activation and functional potentials

尽管经过了几十年的研究，成功的HIV-1疫苗的开发还没有 已实现。因此，需要对激活的淋巴细胞的功能有更好的了解。长期的 我们研究的目的是了解HIV-1包膜特异性免疫细胞的全部潜力。 CD4+T细胞通过支持8-细胞产生抗体而参与HIV-1的控制 CD8+T细胞的激活/维持。然而，根据我们最近的数据，似乎特定于信封 另外，CD4+T细胞可能对病毒感染细胞的控制起到直接作用，而不依赖于8细胞或 CD8+T细胞活性。这里提出的研究将确定这些CD4+T细胞如何赋予它们的“保护者” 效果。 特定目的：确定HIV-1的表型、细胞因子分泌能力和杀伤能力 包膜特异的CD4+T细胞在没有8-细胞或 CD8+T细胞功能。 实验的目的是填补我们对病毒是如何由 免疫系统。这些实验的结果可能对构建新的、成功的 HIV-1疫苗旨在捕捉免疫反应的全部潜力。

Despite decades of research, the development of a successful HIV-1 vaccine has not yet been achieved. A better understanding of the functions of activated lymphocytes is therefore desired. The long-term objective of our research is to comprehend the full potentials of HIV-1-envelope-specific immune cells. CD4+ T-cells contribute to HIV-1 control by supporting antibody production by 8-cells and the activation/maintenance of CD8+ T-cells. However, based on our recent data, it appears that envelope-specific CD4+ T-cells may additionally contribute directly to the control of virus-infected cells, independent of 8-cell or CD8+ T-cell activity. The studies proposed here will determine how these CD4+ T-cells confer their 'protector' effect. Specific Aim: To determine the phenotype, cytokine secretion capacities, and killer potentials of the HIV-1 envelope-specific CD4+ T-cells that protect against envelope-recombinant virus in the absence of 8-cell or CD8+ T-cell functions. Experiments are designed to fill fundamental gaps in our understanding of how virus is controlled by the immune system. Results from these experiments may be invaluable to the construction of new, successful HIV-1 vaccines designed to capture the full potentials of the immune response.