HIV-envelope-specific CD4+ T-cell activation and functional potentials

尽管进行了数十年的研究，但成功的HIV-1疫苗的开发尚未完成。 办妥了一批因此，需要更好地了解活化淋巴细胞的功能。长期 我们研究的目的是了解HIV-1病毒特异性免疫细胞的全部潜力。 CD 4 + T细胞通过支持δ细胞产生抗体来控制HIV-1， 活化/维持CD 8 + T细胞。然而，根据我们最近的数据， CD 4 + T细胞可能另外直接有助于控制病毒感染的细胞，不依赖于8-细胞或 CD 8 + T细胞活性。这里提出的研究将确定这些CD 4 + T细胞如何赋予它们的“保护者”。 效果 具体目的：确定HIV-1的表型、细胞因子分泌能力和杀伤潜力。 在缺乏8-细胞的情况下保护免于HIV重组病毒的HIV特异性CD 4 + T细胞或 CD 8 + T细胞功能。 实验的目的是填补我们对病毒是如何被控制的理解的根本空白。 免疫系统这些实验的结果对于建造新的、成功的 HIV-1疫苗旨在捕捉免疫反应的全部潜力。

Despite decades of research, the development of a successful HIV-1 vaccine has not yet been achieved. A better understanding of the functions of activated lymphocytes is therefore desired. The long-term objective of our research is to comprehend the full potentials of HIV-1-envelope-specific immune cells. CD4+ T-cells contribute to HIV-1 control by supporting antibody production by 8-cells and the activation/maintenance of CD8+ T-cells. However, based on our recent data, it appears that envelope-specific CD4+ T-cells may additionally contribute directly to the control of virus-infected cells, independent of 8-cell or CD8+ T-cell activity. The studies proposed here will determine how these CD4+ T-cells confer their 'protector' effect. Specific Aim: To determine the phenotype, cytokine secretion capacities, and killer potentials of the HIV-1 envelope-specific CD4+ T-cells that protect against envelope-recombinant virus in the absence of 8-cell or CD8+ T-cell functions. Experiments are designed to fill fundamental gaps in our understanding of how virus is controlled by the immune system. Results from these experiments may be invaluable to the construction of new, successful HIV-1 vaccines designed to capture the full potentials of the immune response.