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Structural Biology of sulfotransferase and glycosyltransferase

Structural Biology of sulfotransferase and glycosyltransferase
磺基转移酶和糖基转移酶的结构生物学
批准号：
7593958
负责人：
MASAHIKO NEGISHI
金额：
$38.81万
依托单位：
NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/7593958
关键词：
AffectAffinityAromataseBindingBinding ProteinsBiologicalBiological ProcessBlood coagulationCancer PatientChondroitinChondroitin SulfatesClinicalCommunicationCrystallographyDiseaseDrug Metabolic DetoxicationEnzymesEstrogensGenesGoalsGolgi ApparatusHIVHomeostasisInfectionInorganic SulfatesMembraneMethodsMolecularPharmaceutical PreparationsPlayPolysaccharidesProtein-Carbohydrate InteractionProteinsReactionRecombinant ProteinsResearchRoentgen RaysRoleSignaling MoleculeSiteSolutionsSpecificityStructureSulfatasesSystemTitrationsUnspecified or Sulfate Ion Sulfatesbasecell growthfrontierglycosyltransferasemalignant breast neoplasmmicrobialoutcome forecaststeroid hormonestructural biologysugarsulfotransferasesynthetic enzymethree dimensional structure

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中文摘要
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英文摘要
Cytosolic STs sulfate xenochemicals for detoxification and secretion and also sulfate biological signal molecules such as steroid hormones and bioamines for inactivation and/or storage. Certain sulfated products become carcinogenic. Golgi-membrane STs display high specificity to sulfate distinct sites of polysaccharides. Various glycosyltransferases synthesize polysaccharides and provide STs with substrates. Our research objectives are to eluciadte structure and function of these enzymes. For this, we have used bacterial expression system to produce a large amount of recombinant proteins, have crystallized them, and have solved their 3-dimensional structures. Also Isothermal titration carlimetry is used to investigate various molecular interactactions in solution.
期刊论文(10)
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会议论文
Two-step mechanism that determines the donor binding specificity of human UDP-N-acetylhexosaminyltransferase.
确定人 UDP-N-乙酰己糖胺转移酶供体结合特异性的两步机制。
DOI： 10.1074/jbc.m413379200
发表时间： 2005
期刊： The Journal of biological chemistry
影响因子： --
作者： [Sobhany,Mack, Dong,Jian, Negishi,Masahiko]
通讯作者： Negishi,Masahiko
Phenobarbital and Insulin Reciprocate Activation of the Nuclear Receptor Constitutive Androstane Receptor through the Insulin Receptor.
苯巴比妥和胰岛素通过胰岛素受体相互激活核受体组成型雄甾烷受体。
DOI： 10.1124/jpet.116.232140
发表时间： 2016
期刊： The Journal of pharmacology and experimental therapeutics
影响因子： --
作者： [Yasujima,Tomoya, Saito,Kosuke, Moore,Rick, Negishi,Masahiko]
通讯作者： Negishi,Masahiko
Discovery of estrogen sulfotransferase inhibitors from a purine library screen.
DOI： 10.1021/jm010171u
发表时间： 2001-07
期刊： Journal of medicinal chemistry
影响因子： 7.3
作者： [Dawn E. Verdugo;M. Cancilla;Xue Ge;Nathanael S. Gray;Young-Tae Chang;Peter G. Schultz;Masahiko Negishi-Masahi]
通讯作者： Dawn E. Verdugo;M. Cancilla;Xue Ge;Nathanael S. Gray;Young-Tae Chang;Peter G. Schultz;Masahiko Negishi-Masahi
Statin-activated nuclear receptor PXR promotes SGK2 dephosphorylation by scaffolding PP2C to induce hepatic gluconeogenesis.
DOI： 10.1038/srep14076
发表时间： 2015-09-22
期刊： Scientific reports
影响因子： 4.6
作者： [Gotoh S, Negishi M]
通讯作者： Negishi M
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