Highly Fluorescent Magnetic Nanoprobes for Enhanced Cancer Imaging and Therapy

描述（由申请人提供）：纳米技术被认为有可能从根本上加速癌症的预防、诊断和治疗。由于许多生物学上重要的过程，包括导致癌症的事件，都发生在纳米尺度上，因此在纳米尺度上探测分子过程以及与探针和设备的相互作用非常重要。该提案的广泛目标是开发具有上级光学和磁性的新的多功能纳米探针，用于增强分子癌症成像和治疗。这些新的纳米探针不仅将促进癌症的多模态荧光和磁共振成像，而且还将实现磁场梯度导向的药物靶向和磁场引导的癌症成像。这项拟议中的研究将集中在困扰美国妇女的最常见癌症-乳腺癌，但纳米探针和开发的技术也可能扩展到其他疾病。具体目标是：1）开发创新方法来合成荧光磁性纳米探针，与商业和其他已建立的磁性或荧光探针相比，具有最佳的磁性和光学性质。2)生物学修饰的荧光磁性纳米探针用于人乳腺癌细胞系的特异性靶向。3)设计一种电磁装置，其产生可控的磁场梯度，使得荧光磁性纳米探针能够局部靶向和循环，用于高效的癌症成像和治疗应用。4)使用小鼠异种移植模型对HFMN的靶向、成像和治疗功效进行体内评价，并证明通过外部磁场梯度控制增强的靶向功效。实现这些目标将有助于发现高效的分子纳米探针，以促进癌症的早期检测和治疗。

DESCRIPTION (provided by applicant): Nanotechnology is believed to have the potential to radically accelerate the prevention, diagnosis and treatment of cancer. As many biologically important processes, including events that lead to cancer, happen at the nanometer scale, it is important to probe the molecular processes and interactions with probes and devices at the nanoscale. The broad objective of this proposal is to develop new multifunctional nanoprobes with superior optical and magnetic properties for enhanced molecular cancer imaging and therapy. These new nanoprobes will not only facilitate multimodality fluorescence and magnetic resonance imaging of cancer, but also will enable magnetic field gradient directed drug targeting and magnetic field guided cancer imaging. The proposed research will focus on the most common cancer afflicting American women - breast cancer, but the nanoprobes and the technologies developed may also be extended for other diseases. The specific aims are: 1) Develop innovative approaches to synthesize fluorescent magnetic nanoprobes with both optimal magnetic and optical properties as compared with commercial and other established magnetic or fluorescent probes. 2) Biologically modify the fluorescent magnetic nanoprobes for specific targeting of human breast cancer cell lines. 3) Design an electro-magnetic device which produces a controllable magnetic field gradient that enables localized targeting and circulation of the fluorescent magnetic nanoprobes for highly effective cancer imaging and therapeutic applications. 4) In vivo evaluation of targeting, imaging and therapy efficacy of HFMN using mouse xenograft model, and demonstrate the enhanced targeting efficacy by external magnetic field gradient control. Achieving these aims will contribute to the discovery of highly effective molecular nanoprobes to promote early detection and treatment of cancer.