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Impact of lupus susceptibility loci on peripheral tolerance and onset of disease

狼疮易感位点对外周耐受性和疾病发作的影响

基本信息

批准号：
7669145
负责人：
Ziaur Rahman
金额：
$ 7.73万
依托单位：
THOMAS JEFFERSON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-08-01 至 2011-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7669145
关键词：
Animal Model Antibodies Antibody Formation Antigen Receptors Apoptosis Applications Grants Autoantibodies Autoantigens Autoimmune Diseases B-Cell Development B-Lymphocytes Binding Bone Marrow C57BL/6 Mouse Cells Chromatin Chronic Data Defect Deposition Development Disease Exhibits Fc Receptor Frequencies Genes Genome Genomics Haptens Human Hybrids Immune response Immunoglobulin G Immunoglobulin Somatic Hypermutation Individual Inflammation Kidney Knock-in Mouse Lead Lupus Lymphoid Lymphoid Follicle Mediating Memory Modeling Mus Myelogenous Myeloid Cells Names New Zealand Normal tissue morphology Nuclear Onset of illness Organ Pathogenesis Pathology Pathway interactions Peripheral Phenotype Predisposition Process Production Proteins Publishing Receptors, Antigen, B-Cell Recruitment Activity Regulation Rheumatoid Arthritis Role SLEB1 gene SLEB2 gene SLEB3 gene SLEB5 gene Staging Structure of germinal center of lymph node Surface Susceptibility Gene Systemic Lupus Erythematosus T-Lymphocyte Testing Therapeutic Tissues aged anti-IgG arsonate autoreactive B cell autoreactivity body system congenic ds-DNA insight large scale production mouse model novel diagnostics receptor research study response systemic autoimmune disease

项目摘要

DESCRIPTION (provided by applicant): The hallmark of autoimmune diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) is the production of antibodies that bind to normal tissues (autoantibodies) and, as a result are deposited in multiple organ systems in the body, resulting in inflammation and tissue destruction. Although healthy individuals have the potential to produce autoantibodies, they do not, suggesting that autoantibody producing cells are silenced by a mechanism called tolerance. Most of our understanding of the pathogenesis of autoimmune diseases such as SLE to date has come from the studies of animal models. One such model is the hybrid mouse of New Zealand Black (NZB) x White (NZW) which develop a disease that resembles human SLE. Multiple genes contribute to the pathogenesis of autoimmune diseases such as SLE in humans. Likewise, three major regions of the genome (named Sle1, Sle2 and Sle3/Sle5) containing the genes that contribute to the disease process were identified in this SLE mouse model. Mice containing each of these genomic intervals give rise to different component phenotypes (manifestations) such as autoantibody production, kidney pathology etc., while their interaction culminates in full-blown SLE. It is not well understood how these lupus susceptibility genes are altering B cell tolerance, allowing autoantibody production. Using mouse models producing a high frequency of defined, autoreactive B cells, in the current grant application we propose to study how each of these lupus susceptibility loci influence B cell tolerance and autoantibody production leading to the development of autoimmune disease SLE. This will allow us to identify the defective tolerance processes causing the disease. Subsequently, once the genes in these genomic intervals are identified, we can study how the products of these genes specifically alter the functioning of the identified B cell tolerance processes. Such a mechanistic understanding will allow novel diagnostic and therapeutic approaches for systemic autoimmune diseases to be developed.

描述(由申请人提供)：系统性红斑狼疮(SLE)和类风湿性关节炎(RA)等自身免疫性疾病的特征是产生与正常组织结合的抗体(自身抗体)，结果是沉积在体内的多个器官系统，导致炎症和组织破坏。尽管健康的人有产生自身抗体的潜力，但他们没有，这表明产生自身抗体的细胞被一种称为耐受的机制所抑制。到目前为止，我们对自身免疫性疾病(如SLE)发病机制的大部分了解都来自于动物模型的研究。其中一个这样的模型是新西兰黑(NZB)×白(NZW)的杂交小鼠，它会患上一种类似人类SLE的疾病。多基因参与了自身免疫性疾病的发病机制，如人类系统性红斑狼疮。同样，在这个SLE小鼠模型中，发现了基因组的三个主要区域(命名为Sle1、Sle2和Sle3/Sle5)，其中包含与疾病过程有关的基因。含有这些基因组间隔的小鼠会产生不同的成分表型(表现)，如自身抗体产生、肾脏病理等，而它们的相互作用最终会导致完全发展的系统性红斑狼疮。目前还不清楚这些狼疮易感基因如何改变B细胞的耐受性，从而允许自身抗体的产生。利用产生高频率定义的、自身反应性B细胞的小鼠模型，在当前的赠款申请中，我们建议研究这些狼疮易感基因座如何影响B细胞耐受性和自身抗体的产生，从而导致自身免疫性疾病SLE的发展。这将使我们能够识别导致疾病的有缺陷的耐受过程。随后，一旦确定了这些基因组区间中的基因，我们就可以研究这些基因的产物如何特异性地改变已识别的B细胞耐受过程的功能。这种机械性的理解将使系统性自身免疫性疾病的新诊断和治疗方法得以开发。