REDOX MODULATION OF MUSCLE FUNCTION IN MICROGRAVITY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 具体目的：评估NAC作为人类握力疲劳的对策。N-乙酰半胱氨酸(NAC；还原型硫醇供体)在最近的几项研究中被证明可以抑制人类肌肉疲劳。在健康志愿者身上进行的实验将优化口服NAC的准备和剂量，并将测试NAC在向心运动中对握力和握力疲劳的影响。假设1.口服NAC可以提高血液中的硫醇水平，抑制运动引起的氧化应激，且无不良副作用。将对五种NAC剂量和两种制剂(溶液或胶囊)进行测试，以优化谷胱甘肽保护并将副作用降至最低。假设2.NAC预处理延缓了同心握力练习中的任务失败。Expt提供的最有效的NAC管理协议。1将在标准化的意志力握力练习中测试其延缓疲劳的能力。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Specific Aim: To evaluate NAC as a countermeasure for handgrip fatigue in humans. N-acetylcysteine (NAC; reduced thiol donor) has been shown to inhibit human muscle fatigue in several recent studies. Experiments in healthy volunteers will optimize the preparation and dosage of oral NAC administration and will test NAC effects on handgrip strength and handgrip fatigue during concentric exercise. Hypothesis 1. Oral NAC administration can increase blood thiol levels and inhibit exercise-induced oxidative stress without undesirable side effects. Five NAC doses and two preparations, solution or capsule, will be tested to optimize glutathione protection and minimize side effects. Hypothesis 2. NAC pretreatment delays task failure during concentric handgrip exercise. The most effective NAC administration protocol from Expt. 1 will be tested for its capacity to delay fatigue during a standardized protocol of volitional handgrip exercise.