SIGNAL TRANSDUCTION CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这一核心的目的是为有兴趣研究细胞转导和信号通路的研究人员提供设计、制造表达荧光融合蛋白的载体以进行细胞内定位和蛋白质运输的技术援助。从荧光标记蛋白质的研究中观察到的典型情况是蛋白质的稳态分布，但它们不能直接提供关于分子动力学性质的信息。购买尼康单分子成像系统，用于在37摄氏度孵化时对单细胞进行延时摄影，现已完全配备超形态软件和功能，用于活细胞成像研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this core is to provide technical assistance in designing, making vectors expressing fluorescent fusion proteins for intracellular localization and protein trafficking to investigators interested in studying cellular transduction and signaling pathways. Observations from studies with fluorescently tagged proteins typically show the steady state distribution of a protein, but they do not directly provide information about the kinetic properties of molecules. Acquisition of a Nikon single molecule imaging system equipped for time lapse photography of single cells while under incubation at 37 degrees C has been purchased and is now fully equipped with Metamorph software and functional for live cell imaging studies.