SIGNAL TRANSDUCTION CORE

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 该核心的目的是提供具有共焦特性的落射荧光显微镜。 （目前）向有兴趣研究细胞转导和信号传导途径的研究人员免费提供设备设计和使用方面的技术援助。 对荧光标记蛋白质的研究观察通常显示蛋白质的稳态分布，但它们不直接提供有关分子动力学特性的信息。已购买尼康单分子成像系统，该系统配备用于在 37 摄氏度孵育时对单细胞进行延时摄影，并且现已完全配备 Metamorph 软件，可用于活细胞成像研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this core is to provide access to an epifluorescence microscope with characteristics of a confocal. Technical assistance in the design and utilization of the equipment is (currently) provided free of charge to investigators interested in studying cellular transduction and signaling pathways. Observations from studies with fluorescently tagged proteins typically show the steady state distribution of a protein, but they do not directly provide information about the kinetic properties of molecules. Acquisition of a Nikon single molecule imaging system equipped for time lapse photography of single cells while under incubation at 37 degrees C has been purchased and is now fully equipped with Metamorph software and functional for live cell imaging studies.