Maintenance and Expansion of the TIGR Annotation Engine Service

描述（由申请人提供）：TIGR已经开发了用于原核基因组的手动注释的重要基础设施和工具集。随着测序成本的下降和新的测序技术的出现，小型研究小组或单个实验室获得其感兴趣的生物体的基因组序列已经变得可行。因此，现在有一组重要的研究人员希望手动注释基因组序列，但他们没有所需的基础设施，工具或注释专业知识。自2002年以来，TIGR一直为研究人员提供TIGR注释基础设施和注释引擎服务工具。该服务包括用户提交的基因组的自动注释和手动注释工具Manatee，两者都是完全免费的。此外，我们还提供为期3天的关于注释流程和工具的单独培训课程。我们的努力取得了巨大成功;我们的用户对服务表示高度满意，我们的用户群继续扩大。我们目前有55个用户组，他们总共提交了86个基因组。平均而言，每个月有2-3个新用户组加入该计划，经验丰富的用户继续向我们发送更多的基因组。有了这个应用程序，我们寻求资金，继续免费提供注释引擎服务的研究社区。我们还计划增加几项增强功能，包括：在Manatee中构建序列编辑工具;提供单个蛋白质的搜索更新;让用户可以选择将其数据保留在TIGR的数据库中，并通过互联网访问它和Manatee;扩展Manatee平台，包括Mac OS X和Windows;导出GenBank登录构建器;导出我们的基因本体论注释管理程序;将我们的多基因组注释工具并入Manatee;并导出我们的工作流作业调度系统。这项工作与NIH在公共卫生领域的使命相关，因为注释引擎服务为研究影响人类健康的生物体的研究人员带来了基因组科学的工具。

DESCRIPTION (provided by applicant): TIGR has developed a significant infrastructure and tool set for the manual annotation of prokaryotic genomes. As sequencing costs have dropped and new sequencing technology has become available, it has become feasible for small research groups or single laboratories to obtain a genome sequence of their organism of interest. Therefore, there is now a significant group of researchers who have genome sequences they wish to manually annotate but who do not have the infrastructure, tools, or annotation expertise they need. Since 2002 TIGR has been providing researchers with access to the TIGR annotation infrastructure and tools with the Annotation Engine service. Included in the service is automated annotation of the user-submitted genome and the manual annotation tool Manatee, both completely free-of-charge. In addition, we offer a separate 3-day training course on our annotation processes and tools. Our efforts have met with great success; our users indicate high levels of satisfaction with the service, and our user base continues to expand. We currently have 55 user groups who have submitted a total of 86 genomes. On average, 2-3 new user groups join the program each month and seasoned users continue to send us additional genomes. With this application, we seek funds to continue to provide the Annotation Engine service free-of-charge to the research community. We also plan to add several enhancements, including: build a sequence editing tool into Manatee; provide search updates for individual proteins; give users the option of leaving their data in TIGR's databases and accessing it and Manatee over the internet; extend Manatee platforms to include Mac OS X and Windows; export a GenBank accession builder; export our Gene Ontology annotation management programs; incorporate into Manatee our multiple genome annotation tool; and, export our Workflow job scheduling system. This work is relevant to the mission of the NIH in public health because the Annotation Engine service brings the tools of genomic science to the researchers working on organisms that impact human health.