EFFECT OF A2-ANTIPLASMIN POLYMORPHISM ON CLOTTING MEASURES

这个子项目是众多研究子项目之一

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this research project is to examine the frequency of a Single Nucleotide Polymorphism (SNP) within the gene for a2-antiplasmin (a2-AP). This SNP results in a single amino acid change from arginine (R) to tryptophan (W) at position 6 of the long form of a2-AP. Our aim is to determine the frequency of the SNP in the general population, and to determine how this affects a2-AP levels, protein sequence, and in vitro clotting measures. 300 donors representing the general population will be recruited. The majority of these donors is expected to be OUHSC employees, students, or visitors to campus and will be recruited with an advertisement posted on campus by Dr. Christiansen. The participants will be asked to come to the GCRC between 8 and 10 a.m. and be fasting for at least 8 hours before the blood draw. After obtaining informed consent, height and weight will be measured and recorded, then a physician, nurse or trained phlebotomist will utilize sterile technique to draw 20 ml of blood by venipuncture into ACD anticoagulant. Following the blood draw, a snack will be provided to participants. DNA will be isolated from 1 ml of the blood. PCR techniques will be used to amplify the portion of the a2-APpro encompassing the polymorphism. The genotype will be determined by sequencing of the PCR product. The remainder of the blood will be centrifuged and the plasma collected and frozen for determination of a2-AP levels, protein sequence, a2-APpro-cleaving enzyme activity and for in vitro clotting studies. Specific Aims The purpose of this research project is to examine the frequency of a Single Nucleotide Polymorphism (SNP) within the gene for 2-antiplasmin (2-AP). This SNP results in a single amino acid change from arginine (R) to tryptophan (W) at position 6 of the long form of 2-AP. Our aim is to determine the frequency of the SNP in the general population, and to determine how this affects a2-AP levels, protein sequence, and in vitro clotting measures.