SCIENCE AND RESEARCH CORE

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Science Research Core provides instrumentation and expertise in functional genomics in support of research projects at Maine INBRE member institutions. The Core supports activities in cell and molecular biology, electrophysiology, and high resolution imaging. During the reporting period, the Core provided services in cell culture, flow cytometry and cell line development, confocal microscopy, DNA sequencing, RNA purification and analysis, and gene expression analysis via quantitative PCR. GenBank submission of expressed sequence tags for normalized mixed tissue cDNA libraries of marine model species is in progress, including shark, skate, killifish, green crab, and American lobster. In the past year, newly-designed oligonucleotide microarrays have been produced for killifish, green crab, and lobster for comparative gene expression analysis. Novel cell cultures developed for comparative genomic models include those from adult tissues or embryos of shark, skate and several species of pufferfish. Cell lines also have been developed from zebrafish, platyfish and other freshwater fish model species. The Core has further enhanced biomedical research by establishing transfection technology for introduction of genes into a number of the comparative models used in the Maine INBRE project. The Science Research Core also provided training and individual consultation in cell biology and functional genomics methodology and publishes a set of common molecular biological protocols. In addition, the Core supports the teaching program of Maine INBRE by providing undergraduate and graduate student instruction and postdoctoral training as well as instrumentation and services.