SCIENCE AND RESEARCH CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 科学研究核心提供功能基因组学的仪器和专业知识，以支持缅因州INBRE成员机构的研究项目。核心支持细胞和分子生物学、电生理学和高分辨率成像方面的活动。在本报告所述期间，核心提供了细胞培养、流式细胞术和细胞系开发、共聚焦显微镜、DNA测序、RNA纯化和分析以及通过定量聚合酶链式反应进行基因表达分析的服务。海洋模式物种标准化混合组织cDNA文库的表达序列标签的GenBank提交工作正在进行中，这些海洋模式物种包括鲨鱼、滑板鱼、剑鱼、青蟹和美国龙虾。在过去的一年里，新设计的寡核苷酸微阵列已经被生产出来，用于鲤鱼、青蟹和龙虾的比较基因表达分析。为比较基因组模型开发的新细胞培养物包括鲨鱼、溜冰鱼和几种河豚的成年组织或胚胎。还从斑马鱼、贝壳鱼和其他淡水鱼模式物种中培育出细胞系。该中心通过建立基因导入缅因州INBRE项目中使用的一些比较模型的转基因技术，进一步加强了生物医学研究。科学研究中心还提供细胞生物学和功能基因组学方法学方面的培训和个人咨询，并出版了一套共同的分子生物学方案。此外，中心还通过提供本科生和研究生指导、博士后培训以及仪器和服务来支持缅因州INBRE的教学计划。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Science Research Core provides instrumentation and expertise in functional genomics in support of research projects at Maine INBRE member institutions. The Core supports activities in cell and molecular biology, electrophysiology, and high resolution imaging. During the reporting period, the Core provided services in cell culture, flow cytometry and cell line development, confocal microscopy, DNA sequencing, RNA purification and analysis, and gene expression analysis via quantitative PCR. GenBank submission of expressed sequence tags for normalized mixed tissue cDNA libraries of marine model species is in progress, including shark, skate, killifish, green crab, and American lobster. In the past year, newly-designed oligonucleotide microarrays have been produced for killifish, green crab, and lobster for comparative gene expression analysis. Novel cell cultures developed for comparative genomic models include those from adult tissues or embryos of shark, skate and several species of pufferfish. Cell lines also have been developed from zebrafish, platyfish and other freshwater fish model species. The Core has further enhanced biomedical research by establishing transfection technology for introduction of genes into a number of the comparative models used in the Maine INBRE project. The Science Research Core also provided training and individual consultation in cell biology and functional genomics methodology and publishes a set of common molecular biological protocols. In addition, the Core supports the teaching program of Maine INBRE by providing undergraduate and graduate student instruction and postdoctoral training as well as instrumentation and services.