SCIENCE AND RESEARCH CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 科学研究中心在以下领域为INBRE研究人员和其他人员提供服务和设施：DNA测序和分析、基因表达、生物成像、细胞培养和包括卵母细胞表达在内的电生理学。在某些情况下，核心人员提供单独或正式课程的培训；在其他情况下(例如DNA测序)，服务由核心工作人员提供。目前，与堆芯相关的仪器被安置在几座实验室大楼的可用空间中。2008年6月，其中一些核心设施将合并到目前在建的一座15 000平方英尺的新实验室大楼的两层楼中。 科学研究核心的目标是在每个核心领域保持最先进的仪器和材料资源。为了实现这一目标，除INBRE外，还寻求提供资金的机会，以增加和扩大INBRE的支持。例如，在过去的一年里，收到了外部资金，用于用新的共聚焦荧光显微镜扩大成像核心，并在一个已经很大的数据库中增加大量新的表达序列标签。微阵列应用目前由NSF、NIH和大西洋加拿大机会局提供资金。为了取代2001年用NSF基金购买的老化的定量PCR仪器，2008年1月通过INBRE计划增加了一个新的Stratagene 30005P定量序列检测系统。INBRE资助的初级教职员工和学生将广泛使用QPCR股。 材料资源，包括标准化的cDNA文库、文库克隆和BAC，免费提供给INBRE计划内的研究人员，并向计划外的研究人员收取象征性费用。提供微阵列是为了支付打印成本。 在INBRE计划的下一年，科学研究核心中心将继续为缅因州INBRE社区以及那些可以从其设施和资源中受益的计划外的人提供高效和具有成本效益的服务。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Science Research Core provides services and facilities to INBRE investigators and others in the following areas: DNA sequencing and analysis, gene expression, biological imaging, cell culture, and electrophysiology including oocyte expression. In some cases, the core offers training either individually or in formal courses; in other cases (e.g. DNA sequencing), the service is provided by core staff. At present, instrumentation associated with the core is housed in available spaces in several laboratory buildings. In June 2008, some of these core facilities will be consolidated on two floors of a new 15,000-square-foot laboratory building currently under construction. The goal of the Science Research Core is to maintain state-of-the-art instrumentation and material resources in each of the core areas. To achieve this goal, funding opportunities in addition to INBRE are sought to augment and extend INBRE support. In the past year, for example, external funding was received to expand the imaging core with new confocal fluorescence microscopes and to add a large number of new expressed sequence tags to an already substantial database. Microarray applications are currently funded by NSF, NIH, and the Atlantic Canada Opportunities Agency. To replace an aging quantitative PCR instrument purchased with NSF funds in 2001, a new Stratagene 30005P quantitative sequence detection system was added in January 2008 through the INBRE program. The QPCR unit will be used extensively by INBRE funded junior faculty and students. Material resources including normalized cDNA libraries, library clones, and BACs are provided to researchers within the INBRE program at no charge and for a nominal fee to those outside the program. Microarrays are provided for the cost of printing. In the next year of the INBRE program, the Science Research Core will continue to provide efficient and cost-effective services to the Maine INBRE community as well as those outside the program who can benefit from its facilities and resources.