TBI ELEVATIONS IN INTRACELLULAR CALCIUM CONCENTRATIONS BY NMDA RECEPTORS

NMDA 受体导致 TBI 升高细胞内钙浓度

• 批准号: 7627626
• 负责人: CYNTHIA J GIBSON
• 金额: $ 1.16万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7627626
• 关键词: Acute; Address; Applications Grants; Area; Biomedical Research; Calcium; Chronic; Computer Retrieval of Information on Scientific Projects Database; Data; Data Collection; Educational process of instructing; Elevation; Equipment; Funding; GABA-A Receptor; Grant; Hippocampus (Brain); Injury; Institution; Measures; Mediating; Messenger RNA; N-Methyl-D-Aspartate Receptors; Neurosciences; Process; Productivity; Protein Analysis; Protein Subunits; Proteins; Protocols documentation; Research; Research Infrastructure; Research Personnel; Resources; Schedule; Series; Societies; Source; Time; Tissue Sample; Traumatic Brain Injury; United States National Institutes of Health; Work; brain tissue; falls

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Preliminary data indicate that the GABA-A receptor is altered following experimental traumatic brain injury (TBI) (Gibson & Hamm, in review), however, alterations to GABA-A receptor subunit proteins have yet to be comprehensively characterized. The current research plan is to evaluate which GABA-A receptor subunits are altered by TBI over a series of acute and chronic post-injury time points. Protein (years 1-2) and mRNA (years 4-5) expression of GABA-A receptor subunits will be measured in the hippocampus (an area selectively vulnerable to injury). Also, the proposed calcium-mediated cascade will be manipulated pharmacologically (years 2-3). The time line for this project is on target. As originally indicated in the INBRE grant proposal, the first nine months of the project have primarily involved equipment and protocol set-up. Intense data collection is planned to begin next month. Since my teaching schedule (primary effort allocation during the academic year) was incompatible with the timed projects proposed, the summer months (100% grant effort) will be primarily dedicated to generating brain tissue samples which can then be processed for protein analysis during the next academic year (2005-06). Addressing the productivity of my current INBRE scholar, she has devoted much of her time this year to completing her BRIN (Biomedical Research Infrastructure Network) project, which she has or will soon be presenting to 3 different audiences. We both plan to attend and present at the Society for Neuroscience convention in the fall. During the next (2nd) year of the grant a newly selected INBRE scholar will start working with me on the INBRE project described above.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 初步数据表明，GABA-A受体在实验性创伤性脑损伤（TBI）后发生改变（综述中的吉布森和哈姆），然而，GABA-A受体亚基蛋白的改变尚未得到全面表征。目前的研究计划是评估TBI在一系列急性和慢性损伤后时间点上改变了哪些GABA-A受体亚单位。将在海马体（选择性地易受损伤的区域）中测量GABA-A受体亚单位的蛋白质（第1-2年）和mRNA（第4-5年）表达。此外，将对拟定的钙介导的级联反应进行3年（2-3年）操作。这个项目的时间表是在目标上的。正如INBRE赠款提案中最初指出的那样，该项目的前九个月主要涉及设备和协议设置。计划下个月开始进行密集的数据收集。由于我的教学时间表（主要努力分配在学年）是不兼容的定时项目建议，夏季月份（100%拨款的努力）将主要致力于产生脑组织样本，然后可以处理蛋白质分析在下一学年（2005-06）。 为了提高我目前的INBRE学者的工作效率，她今年花了很多时间来完成她的BRIN（生物医学研究基础设施网络）项目，她已经或即将向3个不同的观众展示。我们都计划参加并出席秋季的神经科学协会大会。在下一个（第二）年的补助金，一个新选择的INBRE学者将开始与我在上述的INBRE项目。