CORE B: CELL SEPARATION & ANALYSIS CORE

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. One overall function of this core is to provide state-of-the-art capabilities for the analysis and separation of cell populations based on physical, immunological, genetic, and other characteristics. These capabilities are of particular importance in working with stem and progenitor cell populations, but also find application in nearly all other types of cell biology research. The Core is equipped with dedicated instruments for the analysis, isolation or depletion of most cell types. These include a Becton Dickinson FACSCalibur flow cytometer, a new FACSAria cell sorter, and a Miltenyi MACS Sorter (Automated Magnetic Cell Sorter). Users are Institute wide, and include investigators in both COBREs (COBRE Vascular Biology and COBRE Stem Cell Biology and Regenerative Medicine). In addition, this core is being developed as a resource for the study of embryonic stem (ES) cells. Technical support, training, and services for stem cell research are provided (especially by Zack Wang). Mouse and NIH-approved human ES cell lines will be maintained with appropriate quality assurance. Technical support provided by the facility will include hands-on instruction in the maintenance, transduction, and differentiation of ES cells. The facility also will provide short-term culture and manipulation of ES cells for individual investigators, and will provide ready access to ES cell reagents (RNAs, genomic DNAs, proteins) without the need to develop specialized technical skills (or space) for ES cell work.