CORE B: CELL SEPARATION & ANALYSIS CORE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。该核心的一个总体功能是为基于物理、免疫、遗传和其他特征的细胞群的分析和分离提供最先进的功能。这些能力在干细胞和祖细胞群体的工作中特别重要，但也在几乎所有其他类型的细胞生物学研究中得到应用。该核心配备了几种仪器，以提供大多数细胞类型的分析，分离或耗尽。这些范围从一个Miltenyi MACS分选机（自动磁性细胞分选机）到四个Becton Dickinson流式细胞仪。最近，一个新的FACS Aria细胞分选系统已获得。目前的参与者包括COBRE血管生物学和COBRE干细胞生物学和再生医学的研究人员。此外，该核心正在开发，以包含胚胎干细胞（ES）研究的资源。提供干细胞研究的技术支持、培训和服务（特别是由Zack Wang提供）。小鼠和美国国立卫生研究院批准的人类胚胎干细胞系将保持适当的质量保证。该机构提供的技术支持将包括在细胞的维护、转导和分化方面的动手指导。该设施还将为个人研究人员提供短期的胚胎干细胞培养和操作，并将为个人研究人员提供胚胎干细胞试剂（rna、基因组dna、蛋白质），而无需为胚胎干细胞工作开发专门的技术技能（或空间）

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. One overall function of this core is to provide state-of-the-art capabilities for the analysis and separation of cell populations based on physical, immunological, genetic, and other characteristics. These capabilities are of particular importance in working with stem and progenitor cell populations, but also find application in nearly all other types of cell biology research. The Core is equipped with several instruments in order to provide the analysis, isolation or depletion of most cell types. These range from a Miltenyi MACS Sorter (Automated Magnetic Cell Sorter) to four Becton Dickinson flow cytometers. Most recently, a new FACS Aria cell sorting system has been acquired. Current participants include investigators in both COBREs (COBRE Vascular Biology and COBRE Stem Cell Biology and Regenerative Medicine). In addition, this core is being developed to comprise a resource for the study of embryonic stem (ES) cells. Technical support, training, and services for stem cell research will be provided (esp by Zack Wang). Mouse and NIH-approved human ES cell lines will be maintained with appropriate quality assurance. Technical support provided by the facility will include hands-on instruction in the maintenance, transduction, and differentiation of the cells. The facility also will provide short-term culture and manipulation of ES cells for individual investigators, and will provide ready access to ES cell reagents (RNAs, genomic DNAs, proteins) for individual investigators without the need to develop specialized technical skills (or space) for ES cell work