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Electroosmotic Pump for Microchip HPLC

Microchip HPLC 电渗泵

基本信息

批准号：
7694295
负责人：
Shaorong Liu
金额：
$ 18.06万
依托单位：
UNIVERSITY OF OKLAHOMA
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-09-30 至 2011-08-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): The concept of lab-on-chip is created with an objective to integrate and perform multiple analytical processes (e.g. sample pretreatment, solution distribution/mixing, separation, detection, etc.) on a microchip platform. So far, most of lab-on-chip research is focused on electrophoretic separations, and relatively much less work is carried out on multi-process integration. One of the main reasons for limited process integration is the lack of a robust and miniaturized pump that is readily integrated with lab-on-chip devices. Microchip HPLC is envisioned to play an important role in point-of-care (POC) measurements and Chemical and Biological Warfare Agent (CBWA) detections. It can be highly parallelized to increase sample throughput (e.g. for drug compound screening), which will have great impact on drug screening and biomarker discovery. Microchip HPLC combined with mass spectrometer could accelerate the proteomic research and reduce its cost considerably. A major challenge toward microchip HPLC is the lack of a robust and miniaturized high-pressure pump that is integrateable with lab-on-chip devices. In availability of reliable on-chip sample injection valves is another obstacle toward these applications. We propose to develop a cascade open-channel EO pump and an on-chip sample injection valve to address these issues. The cascade pump will consist of alternately-arranged "+" and "-" pumps. This pump will be able to generate flow rates of up to 5L/min and pressures of up to 1000 psi for microchip HPLC applications. We will develop a bubbleless electrode to solve the electric connection problems. The electrode will be capable of applying high voltages onto EO pumps while preventing pump solutions from leaking out. In addition, it will minimize the pump solution composition change and eliminate bubble formation due to electrolysis. We will also develop an on-chip sample injection valve for microchip HPLC separations. The valve will have an injection volume ranging from sub-nL to 5L, and the injected volume will be reproducible. All these function-different components will be integrateable with lab-on-chip devices. PUBLIC HEALTH RELEVANCE: We will develop a microchip platform that integrates a cascade open-channel EO pump, an on-chip sample injection valve, a monolith separation column and an on-chip detection scheme to perform HPLC. The cascade pump will consist of alternately-arranged "+" and "-" pumps to generate flow rates of up to 1 5L/min and pressures of up to 1000 psi for microchip HPLC applications. We will develop a bubbleless electrode capable of applying high voltages onto EO pumps while preventing pump solutions from leaking out, and eliminating bubble formation due to electrolysis.

描述（由申请人提供）：创建芯片实验室的概念，目的是集成和执行多个分析过程（例如，样品预处理、溶液分配/混合、分离、检测等）。在一个微芯片平台上。到目前为止，芯片实验室的研究主要集中在电泳分离方面，而在多处理集成方面的研究相对较少。工艺集成有限的主要原因之一是缺乏易于与芯片实验室设备集成的鲁棒且小型化的泵。微芯片HPLC被认为在床旁（POC）测量和化学和生物战剂（CBWA）检测中发挥重要作用。它可以高度并行化以增加样品通量（例如用于药物化合物筛选），这将对药物筛选和生物标志物发现产生重大影响。芯片高效液相色谱与质谱联用可以大大降低蛋白质组学研究的成本。微芯片HPLC的一个主要挑战是缺乏一个强大的和小型化的高压泵，可与芯片实验室设备集成。可靠的片上进样阀的可用性是这些应用的另一个障碍。我们建议开发一个级联的开放通道EO泵和一个芯片上的样品注入阀来解决这些问题。级联泵将由交替布置的“+”和“-”泵组成。该泵将能够产生高达5L/min的流速和高达1000 psi的压力，用于微芯片HPLC应用。我们将开发一种无气泡电极来解决电连接问题。电极将能够向EO泵施加高电压，同时防止泵溶液泄漏。此外，它将最大限度地减少泵溶液成分的变化，并消除由于电解而形成的气泡。我们还将开发一种用于微芯片HPLC分离的片上样品进样阀。阀的进样体积范围为sub-nL至5L，并且进样体积可重现。所有这些功能不同的组件将与芯片实验室设备集成。公共卫生相关性：我们将开发一个微芯片平台，该平台集成了级联开放通道EO泵、片上样品进样阀、整体分离柱和片上检测方案，以执行HPLC。级联泵将由交替布置的“+”和“-”泵组成，以产生高达15 L/min的流速和高达1000 psi的压力，用于微芯片HPLC应用。我们将开发一种无气泡电极，能够在EO泵上施加高电压，同时防止泵溶液泄漏，并消除电解导致的气泡形成。