Electroosmotic Pump for Microchip HPLC

Microchip HPLC 电渗泵

• 批准号: 7589349
• 负责人: Shaorong Liu
• 金额: $ 21.59万
• 依托单位: UNIVERSITY OF OKLAHOMA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-30 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7589349
• 关键词: Address; Biological Markers; Biological Warfare; Blood capillaries; Charge; Chemicals; Chromatography; Compatible; Detection; Devices; Drops; Drug Compounding; Electrodes; Electrolyses; Extravasation; Freezing; Goals; High Pressure Liquid Chromatography; Injection of therapeutic agent; Lead; Liquid substance; Measurement; Microfluidics; Modeling; Peptides; Play; Preclinical Drug Evaluation; Process; Production; Proteins; Proteomics; Public Health; Pump; Range; Rate; Relative (related person); Reporting; Reproducibility; Research; Role; Sampling; Scheme; Screening procedure; Silicon Dioxide; Solutions; Staging; Standards of Weights and Measures; Surface; Technology; Testing; Tubular formation; Work; capillary; concept; cost; design; innovation; mass spectrometer; microchip; miniaturize; novel; point of care; pressure; prevent; voltage

DESCRIPTION (provided by applicant): The concept of lab-on-chip is created with an objective to integrate and perform multiple analytical processes (e.g. sample pretreatment, solution distribution/mixing, separation, detection, etc.) on a microchip platform. So far, most of lab-on-chip research is focused on electrophoretic separations, and relatively much less work is carried out on multi-process integration. One of the main reasons for limited process integration is the lack of a robust and miniaturized pump that is readily integrated with lab-on-chip devices. Microchip HPLC is envisioned to play an important role in point-of-care (POC) measurements and Chemical and Biological Warfare Agent (CBWA) detections. It can be highly parallelized to increase sample throughput (e.g. for drug compound screening), which will have great impact on drug screening and biomarker discovery. Microchip HPLC combined with mass spectrometer could accelerate the proteomic research and reduce its cost considerably. A major challenge toward microchip HPLC is the lack of a robust and miniaturized high-pressure pump that is integrateable with lab-on-chip devices. In availability of reliable on-chip sample injection valves is another obstacle toward these applications. We propose to develop a cascade open-channel EO pump and an on-chip sample injection valve to address these issues. The cascade pump will consist of alternately-arranged "+" and "-" pumps. This pump will be able to generate flow rates of up to 5L/min and pressures of up to 1000 psi for microchip HPLC applications. We will develop a bubbleless electrode to solve the electric connection problems. The electrode will be capable of applying high voltages onto EO pumps while preventing pump solutions from leaking out. In addition, it will minimize the pump solution composition change and eliminate bubble formation due to electrolysis. We will also develop an on-chip sample injection valve for microchip HPLC separations. The valve will have an injection volume ranging from sub-nL to 5L, and the injected volume will be reproducible. All these function-different components will be integrateable with lab-on-chip devices. PUBLIC HEALTH RELEVANCE: We will develop a microchip platform that integrates a cascade open-channel EO pump, an on-chip sample injection valve, a monolith separation column and an on-chip detection scheme to perform HPLC. The cascade pump will consist of alternately-arranged "+" and "-" pumps to generate flow rates of up to 1 5L/min and pressures of up to 1000 psi for microchip HPLC applications. We will develop a bubbleless electrode capable of applying high voltages onto EO pumps while preventing pump solutions from leaking out, and eliminating bubble formation due to electrolysis.

描述（由申请人提供）：创建芯片实验室的概念的目的是在微芯片平台上集成和执行多个分析过程（例如样品预处理、溶液分配/混合、分离、检测等）。目前，芯片实验室的研究大部分集中在电泳分离方面，多工艺集成方面的工作相对较少。工艺集成有限的主要原因之一是缺乏易于与片上实验室设备集成的稳健且小型化的泵。 Microchip HPLC 预计将在现场护理 (POC) 测量和化学和生物战剂 (CBWA) 检测中发挥重要作用。它可以高度并行化以提高样品通量（例如用于药物化合物筛选），这将对药物筛选和生物标志物发现产生巨大影响。 Microchip HPLC与质谱仪相结合可以加速蛋白质组学研究并大大降低其成本。微芯片 HPLC 面临的主要挑战是缺乏可与芯片实验室设备集成的坚固且小型化的高压泵。可靠的片上样品注射阀的可用性是这些应用的另一个障碍。我们建议开发级联开放通道 EO 泵和片上进样阀来解决这些问题。复叠泵由交替排列的“+”和“-”泵组成。该泵能够为微芯片 HPLC 应用产生高达 5L/min 的流速和高达 1000 psi 的压力。我们将开发无气泡电极来解决电连接问题。该电极能够向 EO 泵施加高电压，同时防止泵溶液泄漏。此外，它将最大限度地减少泵溶液成分的变化并消除由于电解而形成的气泡。我们还将开发用于微芯片 HPLC 分离的片上样品注射阀。该阀门的进样量范围为亚nL 至5L，并且进样量可重复。所有这些功能不同的组件都可以与片上实验室设备集成。 公共健康相关性：我们将开发一个微芯片平台，集成级联开放通道 EO 泵、芯片上样品注射阀、整体分离柱和芯片上检测方案以执行 HPLC。级联泵将由交替排列的“+”和“-”泵组成，可为微芯片 HPLC 应用产生高达 1 5L/min 的流速和高达 1000 psi 的压力。我们将开发一种无气泡电极，能够向 EO 泵施加高电压，同时防止泵溶液泄漏，并消除由于电解而形成的气泡。