Neutralization Core

中和核心

• 批准号: 7613727
• 负责人: ELLIS L REINHERZ
• 金额: $ 28.12万
• 依托单位: DANA-FARBER CANCER INST
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7613727
• 关键词: AIDS Vaccines; AIDS vaccine development; Adjuvant; Animals; Antibodies; Antigens; Area; Autologous; Binding; Biological Assay; Cavia; Cholera Toxin; Class; Core Facility; Data; Development; Enzyme-Linked Immunosorbent Assay; Exhibits; Genetic; Glycoproteins; Goals; Granulocyte-Macrophage Colony-Stimulating Factor; HIV-1; Immune Sera; Immune response; Immunization; Immunoglobulin A; Immunoglobulin G; Interleukin-1; Interleukin-12; Mannose; Mineral Oil; Mucosal Immune Responses; Mucosal Immunity; Oryctolagus cuniculus; Preclinical Testing; Production; Proteins; Range; Research Personnel; Ribi adjuvant; Route; SIV; Saline; Sampling; Serum; Source; Squalene; Standards of Weights and Measures; Study of serum; Testing; Vaccines; Vagina; Vaginal Douching; aluminum sulfate; comparative; cross reactivity; cytokine; design; immunogenicity; incomplete Freund' s adjuvant; member; neutralizing antibody; novel; rectal

Core C: Immunization and Neutralization Core This Core will provide critical support to multiple projects of this HIVRAD by providing information on the potency and cross-reactivity of neutralizing antibodies generated by native and novel SIV and HIV-1 envelope glycoproteins. We have assembled a team of outstanding investigators who have combined expertise in all areas of our Core effort. Our goal is to keep pace with the design and production efforts of this HIVRAD by providing timely comparative immunogenicity data. We will conduct irmamnogenicity studies in guinea pigs and rabbits that will include both systemic and mucosal immunity with standard and novel adjuvants. Samples from these animals wilt be assessed for antibodies that neutralize the matched vaccine strain and a wide spectrum of heterologous strains of either SIV or multiple genetic subtypes of HIV-1, as appropriate. In addition, Env-specific binding antibodies will be assessed in serum and vaginal lavages with respect to IgG and IgA classes. The long term goal of this Core C is to determine which immunogens, adjuvants and routes of immunization are capable of inducing neutralizing antibodies that are both potent and broadly-reactive for AIDS vaccine development.

核心C：免疫和中和核心 这一核心将通过提供有关艾滋病毒/艾滋病防治的信息， 天然和新型SIV和HIV-1包膜产生的中和抗体的效力和交叉反应性 糖蛋白我们已经组建了一支优秀的调查人员团队，他们结合了我们所有领域的专业知识， 核心努力。我们的目标是通过提供及时的信息， 比较免疫原性数据。我们将在豚鼠和家兔中进行免疫原性研究，包括 使用标准和新型佐剂的全身和粘膜免疫。将对这些动物的样本进行评估 对于中和匹配的疫苗株和SIV或SIV的广谱异源株的抗体， HIV-1的多种基因亚型。此外，Env特异性结合抗体将在 血清和阴道灌洗液的IgG和伊加类。核心C的长期目标是确定 免疫原、佐剂和免疫途径能够诱导中和抗体， 并且对艾滋病疫苗的开发具有广泛的反应性。