STRUCTURES OF VARIANT CRE RECOMBINASE SYNAPTIC COMPLEXES AND EVALUATION OF ABF2-

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 变异的Cre重组酶-Lox DNA复合体的高分辨率数据(2.0-2.2)将被收集用于结构优化。这些配合物测试了以下假设：i)Cre如何在反应周期的不同时间点识别其天然DNA底物loxP，ii)Cre与突变DNA底物的不同作用如何改变反应活性，以及iii)底物如何促进反应。已经收集了中等分辨率的数据集(2.7-3.4)，证明了这些晶体的实用性。还需要试束时间来评估线粒体DNA包装蛋白ABF2在27聚体DNA络合物中的晶体衍射。这些晶体目前使用Cuka辐射产生3.2A-4.5°的衍射峰。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. High resolution data (2.0-2.2 ¿) for variant Cre recombinase-Lox DNA complexes will be collected for structure refinement. These complexes test hypotheses concerning i) how Cre recognizes its natural DNA substrate LoxP at different points in the reaction cycle, ii) how differential interactions of Cre with mutant DNA substrates alter reactivity and iii) how the substrate may promote the reaction. Moderate resolution data sets (2.7-3.4 ¿) have already been collected, demonstrating the usefulness of these crystals. Trial beam time is also requested to evaluate the diffraction of crystals of a mitochondrial DNA packaging protein, ABF2, in complex with 27mer DNA. These crystals presently diffract 3.2 A- 4.5 ¿ using CuKa radiation.