STRUCTURES OF VARIANT CRE RECOMBINASE SYNAPTIC COMPLEXES AND EVALUATION OF ABF2-

变体CRE重组酶突触复合物的结构和ABF2-的评估

• 批准号: 7370333
• 负责人: ENOCH P BALDWIN
• 金额: $ 0.19万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7370333
• 关键词: STRUCTURES; VARIANT; CRE; RECOMBINASE; SYNAPTIC

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. High resolution data (2.0-2.2 ¿¿ for variant Cre recombinase-Lox DNA complexes will be collected for structure refinement. These complexes test hypotheses concerning i) how Cre recognizes its natural DNA substrate LoxP at different points in the reaction cycle, ii) how differential interactions of Cre with mutant DNA substrates alter reactivity and iii) how the substrate may promote the reaction. Moderate resolution data sets (2.7-3.4 ¿¿ have already been collected, demonstrating the usefulness of these crystals. Trial beam time is also requested to evaluate the diffraction of crystals of a mitochondrial DNA packaging protein, ABF2, in complex with 27mer DNA. These crystals presently diffract 3.2 A- 4.5 ¿¿using CuKa radiation.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。将收集变体Cre重组酶-Lox DNA复合物的高分辨率数据（2.0-2.2）用于结构精修。这些复合物测试关于i）Cre如何在反应循环中的不同点识别其天然DNA底物LoxP，ii）Cre与突变DNA底物的差异相互作用如何改变反应性和iii）底物如何促进反应的假设。已经收集了中等分辨率的数据集（2.7-3.4），证明了这些晶体的有用性。还要求试验光束时间以评估与27聚体DNA复合的线粒体DNA包装蛋白ABF 2晶体的衍射。这些晶体目前使用CuKa辐射的行为为3.2 A- 4.5。