DEVELOPMENT OF CELL-BASED ASSAYS FOR RICIN AND ABRIN TOXINS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 功能性毒素可引起细胞反应(如细胞凋亡)，这种反应可 用流式细胞仪检测。我们将开发基于细胞的分析方法来 确认复杂样品中存在功能性毒素。我们会 鉴定对蓖麻毒素具有快速反应和高敏感性的细胞系 阿比林毒素。因为样本可能含有无毒成分，会引发 类似的细胞反应，特定的中和抗体将被用作 控制。在存在中和毒素的特定抗体的情况下 功能，测量的细胞反应将减少或将成为 检测不到。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Functional toxins elicit cellular responses (e.g. apoptosis) that can be measured by flow cytometry. We will be developing cell-based assays to confirm the presence of functional toxin in complex samples. We will identify cell lines with quick response and high sensitivity to Ricin and Abrin toxins. Because samples may contain non-toxin ingredients that trigger similar cellular responses, specific neutralizing antibodies will be used as controls. In the presence of specific antibodies that neutralize toxin functions, measured cellular response will decrease or will become undetectable.