KCSA REVERSE MICELLE CB EXPERIMENT

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 完整的膜蛋白在多种生物学过程中发挥着重要作用，包括神经动作电位、动态平衡、细胞结构和信号转导。它们占当前和潜在药物靶点的50%以上，但它们的结构特征远远落后于可溶性蛋白质。对于溶液核磁共振波谱，膜蛋白是一个重大的挑战，因为它们在洗涤剂胶束中的增溶作用导致颗粒尺寸较大，并伴随着缓慢的分子取向。这限制了可以使用的光谱学的复杂性。我们开发了一种新的方法，用于在表面活性剂体系中溶解膜蛋白，该体系适用于溶解在低粘度溶剂中，如戊烷、丁烷或丙烷，并正在收集3D数据以进行骨架指定。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Integral membrane proteins play essential roles in a wide array ofbiological processes including nerve action potentials, homeostasis, cellarchitecture, and signal transduction. They comprise over 50% of currentand potential drug targets, yet their structural characterization haslagged far behind that of soluble proteins. For solution nuclear magneticresonance (NMR) spectroscopy, membrane proteins represent a significantchallenge because their solubilization in aqueous detergent micellesresults in particles of large size and attendant slow molecularreorientation. This limits the complexity of spectroscopy that can beemployed. We have developed a novel procedure for solubilizing membrane proteins in a surfactant system suitable for dissolution in low viscosity solvents such as pentane, butane, or propane, and are in the process of collecting 3D data for backbone assignments.