STRUCTURE DETERMINATION OF A BRANCH POINT DOMAIN

分支点域的结构确定

• 批准号: 7598784
• 负责人: R KO SIGEL
• 金额: $ 0.16万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7598784
• 关键词: Bacteria; Catalytic RNA; Computer Retrieval of Information on Scientific Projects Database; Dependence; Eukaryota; Eukaryotic Cell; Exhibits; Funding; Grant; Institution; Introns; Ions; Measures; Metals; Molecular Conformation; Nature; RNA; RNA Splicing; Research; Research Personnel; Residual state; Resources; Source; Spliceosomes; Structure; United States National Institutes of Health; interest; nuclear Overhauser enhancement; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Group II introns are naturally occurring self splicing ribozymes of mainly lower eukaryotes and bacteria, that exhibit a similar catalytic mechanism as the mammalian spliceosome. To decipher the mechanistic similarities and differences to the spliceosome on an atomic level, we are interested in the exact conformation of the branch point in domain 6 of the group II intron ai5g and its dependence on the presence, concentration and nature of metal ions. To this end we are planning to measure residual dipolar couplings as well as 1D NOE experiments of our 39mer RNA hairpin of D6.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 第二组内含子是天然存在的核酶，主要存在于低等真核生物和细菌中，其催化机制与哺乳动物剪接体相似。为了在原子水平上破译与剪接体的机制上的异同，我们感兴趣的是II组内含子AI5g结构域6中分支点的确切构象及其对金属离子的存在、浓度和性质的依赖。为此，我们计划测量剩余的偶极耦合以及我们的39聚RNA发夹D6的一维NOE实验。