STRUCTURE DETERMINATION OF A BRANCH POINT DOMAIN

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Group II introns are naturally occurring self splicing ribozymes of mainly lower eukaryotes and bacteria, that exhibit a similar catalytic mechanism as the mammalian spliceosome. To decipher the mechanistic similarities and differences to the spliceosome on an atomic level, we are interested in the exact conformation of the branch point in domain 6 of the group II intron ai5g and its dependence on the presence, concentration and nature of metal ions. To this end we are planning to measure residual dipolar couplings as well as 1D NOE experiments of our 39mer RNA hairpin of D6.