GROEL VARIANT

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 以前的研究无法捕获和研究GroEL-Groes复合体的预触发状态并不令人惊讶，因为野生型GroEL只能短暂地填充这种变构状态(大约100毫秒)，这大大缩短了准备操作的时间。相比之下，我们修改后的GroEL变体似乎以相当慢的速度通过了这一过渡。我们建议，利用NCMI在用冷冻EM检查像GroEL这样的分子方面取得的技术进步，以高分辨率观察这个新的GroEL变体，可能会提供一个前所未有的GroEL-Groes机器关键变构状态的快照，目前我们对此知之甚少。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The inability of previous studies to trap and study a pre-triggered state of the GroEL-GroES complex is not surprising, given that wild-type GroEL populates this allosteric state only transiently (for a hundred milisec or so), much to short a time for ready manipulation. By contrast, our modified GroEL variant appears to move through this transition at a considerably slower rate. We propose that a high-resolution look at this new GroEL variant, exploiting the technical advances the NCMI has made in examining molecules like GroEL by cryo-EM, is likely to provide an unprecedented snap-shot of a critical allosteric state of the GroEL-GroES machine about which we currently know very little.