GROEL VARIANT

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 以前的研究无法捕获和研究GroEL-Groes复合体的预触发状态并不令人惊讶，因为野生型GroEL只能瞬时地(约100毫秒)填充这种变构状态，对于大多数生化和生物物理操作来说时间太短了。相比之下，我们修改后的GroEL变体进行这种转换的速度似乎要慢得多。我们认为，这种新的GroEL变体的高分辨率结构，利用NCMI在通过冷冻-EM检查GroEL等分子方面取得的技术进步，很可能提供一个前所未有的GroEL-Groes机器关键变构状态的快照，而人们对此知之甚少。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The inability of previous studies to trap and study a pre-triggered state of the GroEL-GroES complex is not surprising, given that wild-type GroEL populates this allosteric state only transiently (for about a hundred millisec), too short a time for most biochemical and biophysical manipulation. By contrast, our modified GroEL variant appears to undergo this transition at a considerably slower rate. We propose that a high-resolution structure of this new GroEL variant, exploiting the technical advances the NCMI has made in examining molecules like GroEL by cryo-EM, is likely to provide an unprecedented snap-shot of a critical allosteric state of the GroEL-GroES machine about which little is known.