ULTRASTRUCTURE OF APOPTOTIC MITOCHONDRIA
ULTRASTRUCTURE OF APOPTOTIC MITOCHONDRIA
批准号:
7601022
负责人:
DONALD DAVID NEWMEYER
金额:
$1.09万
依托单位国家:
美国
项目类别:
财政年份:
2007
资助国家:
美国
项目状态:
已结题
起止时间:
2007-05-01 至 2008-04-30
关键词:
ApoptosisApoptoticBiological PreservationBuffersCarbohydratesCollaborationsComputer Retrieval of Information on Scientific Projects DatabaseCytoplasmFreezingFundingGrantIn VitroInstitutionLiver MitochondriaMannitolMembraneMethodsMitochondriaMusOrganellesOuter Mitochondrial MembranePathway interactionsPreparationProcessProtein FamilyProteinsResearchResearch PersonnelResourcesRuptureSourceStandards of Weights and MeasuresSucroseTimeTrehaloseUnited States National Institutes of HealthWorkcontrolled releaseresearch study
中文摘要
这个子项目是众多研究子项目之一
英文摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
We are investigating a method for the long-term storage of mitochondria in trehalose-containing buffer. In apoptosis, Bcl-2-family proteins regulate the barrier function of the mitochondrial outer membrane (MOM), controlling the release of proapoptotic proteins from the intermembrane space (IMS) into the cytoplasm. This process can be studied in vitro with freshly isolated mouse liver mitochondria. Unfortunately, mitochondria frozen/thawed in standard sucrose-mannitol buffers become leaky and useless for apoptosis research. Much of what we know about the mitochondrial apoptotic pathway has been determined from experiments using isolated mouse liver mitochondria. The outer membranes of these isolated organelles tend to deteriorate over time and rupture immediately upon freezethaw. Thus, the functional analysis of mouse liver mitochondria (and presumably mammalian mitochondria from other sources) currently requires freshly prepared organelles. To reduce the need for costly and timeconsuming preparations, we sought to identify a storage medium that preserves mitochondrial function, particularly of the outer membrane. To that end, we analyzed the integrity of MOMs after rapid freezethaw in buffers containing various carbohydrates. Part of this analysis was to determine the structural preservation of isolated mitochondria. This work was done at the NCMIR in collaboration with Dr. Perkins.
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ACTIVATION OF MITOCHONDRIAL OUTER MEMBRANE PERMEABILIZATION BY BH3-ONL
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批准号:8169608
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项目类别:
-
资助金额:$0.72万
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财政年份:2010
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负责人:DONALD DAVID NEWMEYER
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依托单位:
Mitochondria, apoptosis and the Bcl-2 family
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批准号:8077521
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项目类别:
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资助金额:$8.49万
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财政年份:2010
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负责人:DONALD DAVID NEWMEYER
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依托单位:
ACTIVATION OF MITOCHONDRIAL OUTER MEMBRANE PERMEABILIZATION BY BH3-ONL
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批准号:7957616
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项目类别:
-
资助金额:$0.94万
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财政年份:2009
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负责人:DONALD DAVID NEWMEYER
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依托单位:
ACTIVATION OF MITOCHONDRIAL OUTER MEMBRANE PERMEABILIZATION BY BH3-ONL
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批准号:7722439
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项目类别:
-
资助金额:$0.59万
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财政年份:2008
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负责人:DONALD DAVID NEWMEYER
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依托单位:
ACTIVATION OF MITOCHONDRIAL OUTER MEMBRANE PERMEABILIZATION BY BH3-ONL
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批准号:7601097
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项目类别:
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资助金额:$0.22万
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财政年份:2007
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负责人:DONALD DAVID NEWMEYER
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依托单位:
MITOCHONDRIA FROZEN WITH TREHALOSE RETAIN BIOLOGICAL FUNCTIONS
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批准号:7601060
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项目类别:
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资助金额:$1.09万
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财政年份:2007
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负责人:DONALD DAVID NEWMEYER
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依托单位:
MITOCHONDRIA FROZEN WITH TREHALOSE RETAIN BIOLOGICAL FUNCTIONS
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批准号:7358132
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项目类别:
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资助金额:$0.51万
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财政年份:2006
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负责人:DONALD DAVID NEWMEYER
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依托单位:
ULTRASTRUCTURE OF APOPTOTIC MITOCHONDRIA
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批准号:7358055
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项目类别:
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资助金额:$0.51万
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财政年份:2006
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负责人:DONALD DAVID NEWMEYER
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依托单位:
ULTRASTRUCTURE OF APOPTOTIC MITOCHONDRIA
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批准号:7181351
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项目类别:
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资助金额:$0.54万
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财政年份:2005
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负责人:DONALD DAVID NEWMEYER
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依托单位:
ULTRASTRUCTURE OF APOPTOTIC MITOCHONDRIA
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批准号:6975374
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项目类别:
-
资助金额:$1.29万
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财政年份:2004
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负责人:DONALD DAVID NEWMEYER
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依托单位:
Mitochondria, apoptosis and the Bcl-2 family
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批准号:7123844
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项目类别:
-
资助金额:$37.07万
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财政年份:2001
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负责人:DONALD DAVID NEWMEYER
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依托单位:
MITOCHONDRIAL FUNCTION IN APOPTOSIS
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批准号:6636547
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项目类别:
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资助金额:$31.96万
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财政年份:2001
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负责人:DONALD DAVID NEWMEYER
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依托单位:
Mitochondria, apoptosis and the Bcl-2 family
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批准号:7031963
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项目类别:
-
资助金额:$36.16万
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财政年份:2001
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负责人:DONALD DAVID NEWMEYER
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依托单位:
Mitochondria, apoptosis and the Bcl-2 family
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批准号:7281238
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项目类别:
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资助金额:$35.99万
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财政年份:2001
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负责人:DONALD DAVID NEWMEYER
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依托单位:
Bax function in apoptosis
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批准号:8534143
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项目类别:
-
资助金额:$37.86万
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财政年份:2001
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负责人:DONALD DAVID NEWMEYER
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依托单位:
Bax function in apoptosis
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批准号:8042496
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项目类别:
-
资助金额:$39.63万
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财政年份:2001
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负责人:DONALD DAVID NEWMEYER
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依托单位:
MITOCHONDRIAL FUNCTION IN APOPTOSIS
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批准号:6228448
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项目类别:
-
资助金额:$28.2万
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财政年份:2001
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负责人:DONALD DAVID NEWMEYER
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依托单位:
Bax function in apoptosis
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批准号:8142820
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项目类别:
-
资助金额:$39.24万
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财政年份:2001
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负责人:DONALD DAVID NEWMEYER
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依托单位:
MITOCHONDRIAL FUNCTION IN APOPTOSIS
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批准号:6520374
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项目类别:
-
资助金额:$31.96万
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财政年份:2001
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负责人:DONALD DAVID NEWMEYER
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依托单位:
MITOCHONDRIAL FUNCTION IN APOPTOSIS
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批准号:6706331
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项目类别:
-
资助金额:$31.96万
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财政年份:2001
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负责人:DONALD DAVID NEWMEYER
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依托单位:
海外基金