RECONSTRUCTION AND MODELING OF NORMAL AND GENETICALLY ENGINEERED MOUSE HEART

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 使用共聚焦显微镜生成亚微米分辨率的完整小鼠心脏的三维重建是很重要的。目前正在对正常和转基因小鼠的心脏进行图像收集和重建。这些关于非均匀分布的组织属性和整体心脏结构的信息将被用来创建有限元网格，用于对小鼠心脏进行完全耦合的机电模拟。这些模拟将使我们能够预测特定的基因操作对心脏整体机械和电功能的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. It is important to generate three-dimensional reconstructions of whole mouse hearts at sub-micron resolution using confocal microscopy. Image collection and reconstruction is being performed on hearts from both normal and genetically altered mice. This information on heterogeneously distributed tissue properties and overall ventricular structure will be used to create finite element meshes for fully-coupled electromechanical simulations of the mouse heart. These simulations will allow us to predict the effects of specific genetic manipulations on the overall mechanical and electrical function of the heart.