IDENTIFICATION OF CSE4-INTERACTING PROTEINS

CSE4 相互作用蛋白的鉴定

• 批准号: 7602231
• 负责人: Susan Biggins
• 金额: $ 0.56万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602231
• 关键词: Aneuploidy; Cell Cycle; Centromere; Chromatin Structure; Chromosomes; Computer Retrieval of Information on Scientific Projects Database; Congenital Abnormality; DNA; DNA Sequence; Deposition; Epigenetic Process; Funding; Genomic Instability; Grant; Histone H3; Institution; Kinetochores; Localized; Mediating; Nucleosomes; Proteins; Research; Research Personnel; Resources; Saccharomycetales; Site; Source; Specific qualifier value; United States National Institutes of Health; Variant; daughter cell; prevent; protein structure; tumor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During every cell cycle, chromosomes must be accurately partitioned to daughter cells to prevent genomic instability and aneuploidy, a hallmark of all tumors and many birth defects. Chromosomes segregate using their kinetochores, the specialized protein structures that are assembled on centromeric DNA sequences and mediate attachment to the spindle. One hallmark of all eukaryotic kinetochores is an essential centromeric histone H3 (CenH3) variant that localizes exclusively to centromeres and replaces canonical histone H3 in centromeric nucleosomes. Because centromeric DNA sequences are not conserved, CenH3 has been proposed to be the epigenetic component that specifies the site of kinetochore assembly. Although CenH3 is an essential component of all kinetochores and is required for centromeric chromatin structure, little is known about CenH3 incorporation into centromeric DNA. We therefore propose to purify the budding yeast CenH3, Cse4, to identify interacting proteins that may regulate its exclusive deposition at the centromere.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 在每个细胞周期中，染色体必须准确地分配给子细胞，以防止基因组不稳定和非整倍性，这是所有肿瘤和许多出生缺陷的标志。 染色体利用动粒进行分离，动粒是一种特殊的蛋白质结构，组装在着丝粒 DNA 序列上并介导与纺锤体的附着。 所有真核动粒的一个标志是一种重要的着丝粒组蛋白 H3 (CenH3) 变体，它专门定位于着丝粒并取代着丝粒核小体中的典型组蛋白 H3。 由于着丝粒 DNA 序列不保守，CenH3 被认为是指定着丝粒组装位点的表观遗传成分。 尽管 CenH3 是所有着丝粒的重要组成部分，并且是着丝粒染色质结构所必需的，但关于 CenH3 掺入着丝粒 DNA 的情况知之甚少。 因此，我们建议纯化出芽酵母 CenH3、Cse4，以鉴定可能调节其在着丝粒上的唯一沉积的相互作用蛋白。