MAPPING THE STIMULUS-SPECIFIC SIGNALING PATHWAYS IN PERIODONTITIS BY PROTEOMICS

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 牙周炎是一种宿主-寄生虫相互作用的炎症性疾病，导致结缔组织破坏和牙槽骨吸收。牙龈卟啉单胞菌（P.g.）是一种黑色素革兰氏阴性厌氧细菌，是牙周炎发生和进展的主要病原体。细胞壁和附属结构，如脂多糖 (LPS) 和菌毛 (Fim A)，在诱导先天免疫反应中发挥重要作用，包括局部和循环单核细胞/巨噬细胞产生细胞因子。根据初步数据，实时 P.g.刺激人外周血单核细胞 (PBM) 和 THP-1 中独特的促炎信号转导途径，而不是纯化的细菌成分。在这些实验中，我们在蛋白质表达水平上测试了活 P.g.、LPS 和 Fim A 差异诱导的独特信号通路。描述我们方法的开发和首次应用的手稿已出版。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Periodontitis is an inflammatory disease with host-parasite interactions which contribute to connective tissue destruction and alveolar bone resorption. Porphyromonas gingivalis (P.g.) a black-pigmented Gram-negative anaerobic bacterium, is a major pathogen in the development and progression of periodontitis. Cell wall and appendage structures, such as lipopolysaccharide (LPS) and fimbriae (Fim A), play important roles in the induction of innate immune responses, including cytokine production by localized and circulating monocyte/macrophage. Based on preliminary data, live P.g. stimulates unique pro-inflammatory signal transduction pathways in human peripheral blood monocytes (PBM) and THP-1 as opposed to purified bacterial components. In these experiments we test at the level of protein expression that unique signaling pathways are differentially induced by live P.g., LPS and Fim A. A manuscript describing the development and first application of our methodology is in press.