HIGH THROUGHPUT STRUCTURE DETERMINATION OF PROTEIN COMPLEXES

蛋白质复合物的高通量结构测定

• 批准号: 7602321
• 负责人: ZHONGMIN WANG
• 金额: $ 0.36万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602321
• 关键词: Complex; Computer Retrieval of Information on Scientific Projects Database; Data Collection; Data Set; Drug Design; Equipment; Funding; Grant; Home environment; Housing; Institution; Numbers; Pharmacologic Substance; Process; Proteins; Research; Research Personnel; Resources; Roentgen Rays; Source; Structure; Synchrotrons; United States National Institutes of Health; beamline; day

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. New Century Pharmaceuticals, Inc. is going to conduct high throughput crystal structure determination of protien-compound complexes using NSLS synchrotron facility. The strucutre of the native protein is already known. Due to the space group of the crystals (triclinic), it takes about 3 days to collect one dataset on the in-house Rigaku/MSA x-ray equipment. Large number of complexes quickly saturates the capacity of our home sources. Using NSLS beamlines, we are hoping to have quicker turnaround on X-ray data collection. This in turn will significantly increase the throughput and spped up the research process and rational drug design efforts.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 New Century Pharmaceuticals, Inc. 将使用 NSLS 同步加速器设施对蛋白质化合物复合物进行高通量晶体结构测定。天然蛋白质的结构是已知的。由于晶体的空间群（三斜），在内部 Rigaku/MSA X 射线设备上收集一个数据集大约需要 3 天。大量的综合体很快就饱和了我们家庭资源的容量。使用 NSLS 光束线，我们希望能够更快地完成 X 射线数据收集。这反过来将显着提高通量并加快研究过程和合理的药物设计工作。