NEURONAL TURNOVER IN ADULT BRAIN USING AMS FOR RETROSPECTIVE BIRTH DATING CELLS

使用 AMS 进行追溯出生约会细胞的成人大脑神经元周转

基本信息

  • 批准号:
    7602409
  • 负责人:
  • 金额:
    $ 2.85万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2007
  • 资助国家:
    美国
  • 起止时间:
    2007-09-01 至 2008-08-31
  • 项目状态:
    已结题

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Neurogenesis is known to occur in specific regions of the adult animal brain, but the extent and comparability of neurogenesis in the adult human brain is much harder to determine, and to date largely unknown. Traditional methods used for dating cells are limited in the information they provide, or are not appropriate for human use. Thus, currently there is no method available to study cellular turnover in man. We propose to develop a method for the retrospective birth dating of cells. We are interested in using bomb pulse carbon-14 (C14) dating as a method for measuring the approximate age of specific populations of cells in the adult human brain. This method is based on establishing the proportion of the isotope C14 in genomic DNA. C14 measurements will be made using the highly sensitive accelerator mass spectrometer (AMS). After a cell has terminally differentiated it does not divide again. Since the last cell division represents the last time point when the cell synthesized DNA, its chromosomal DNA will reflect the age when the cell was born. Traditionally, the slow decay of C14 relative to other carbon species has given it a temporal resolution of many years, however due to nuclear tests in the late 1950s and early 1960s, the level of C14 in the atmosphere has increased dramatically. This level has since dropped off in an exponential fashion, allowing one to resolve C14 differences in the range of years. Because DNA has a C14 content reflective of the time when it was synthesized, establishing the C14 content of chromosomal DNA will enable us to retrospectively birth date cells, and thus establish cellular turnover. Crucial to the understanding of basic biological processes, is information about cellular turnover. As well as having an interest in normal cellular turnover, many diseases are thought to be affected in their generation of new cells. Information about cellular turnover in disease states may provide novel insights into the pathological processes of the disease, and possibly suggest new therapeutic strategies. Preliminary experiments using AMS to date C14 from horse brain DNA have yielded encouraging results. The next step is to look at how accurately AMS dates C14 from DNA extracted from horse blood, brain and teeth. Particular populations of cells will then be isolated using FACS analysis (which allows specific cell populations to be isolated e.g. one can sort for neurons using neuronal specific markers such as NeuN, ¿ tubulin or Thy1). Once this technique has been established we aim to move onto human material, and study cellular turnover in specific human pathologies. Ethical permission to obtain postmortem human material has been granted. We used this strategy to determine the age of cells in the cortex of the adult human brain, and showed that whereas non-neuronal cells are exchanged, occipital neurons are as old as the individual, supporting the view that postnatal neurogenesis does not take place in this region. Retrospective birth dating is a generally applicable strategy that can be used to measure cell turnover in man under physiological and pathological conditions.
这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者(PI)可能从另一个NIH来源获得了主要资金, 因此可以在其他CRISP条目中表示。列出的机构为 中心,但不一定是研究者所在的机构。 已知神经发生发生在成年动物脑的特定区域,但是神经发生的程度和可比性是未知的。 成年人大脑中的神经发生更难确定,迄今为止基本上是未知的。 传统方法 用于测定细胞年龄的方法所提供的信息有限,或者不适合人类使用。 因此,目前 目前还没有研究人类细胞更新的方法,我们建议开发一种回顾性的方法, 细胞的诞生日期 我们感兴趣的是使用炸弹脉冲碳-14(C14)定年作为测量的方法, 成年人大脑中特定细胞群的近似年龄。 该方法基于建立 同位素C14在基因组DNA中的比例。 C14测量将使用高灵敏度加速器进行 质谱仪(AMS)。 细胞在终末分化后,不再分裂。 因为最后一次细胞分裂代表了 当细胞合成DNA时,其染色体DNA将反映细胞出生时的年龄。 传统上, C14相对于其他碳物种的缓慢衰变使其具有多年的时间分辨率,然而,由于 在20世纪50年代末和60年代初的核试验中,大气中的C14水平急剧增加。 这 此后,C14水平以指数方式下降,使人们能够解决C14在年份范围内的差异。 由于DNA具有反映其合成时间的C14含量,因此确定DNA的C14含量是必要的。 染色体DNA将使我们能够追溯细胞的出生日期,从而建立细胞更新。 了解基本的生物学过程的关键是关于细胞周转的信息。 除了拥有一个 由于对正常细胞更新感兴趣,许多疾病被认为影响了新细胞的生成。 关于疾病状态下细胞周转的信息可能为疾病的病理过程提供新的见解。 疾病,并可能提出新的治疗策略。 利用AMS测定马C14的初步实验 大脑DNA已经取得了令人鼓舞的成果。 下一步是看看AMS从DNA中确定C14的准确性 从马血脑和牙齿中提取 然后使用FACS分析分离特定的细胞群 (其允许分离特定的细胞群,例如,可以使用神经元特异性标记物, 如NeuN、微管蛋白或Thy 1)。 一旦这项技术建立起来,我们的目标是转向人体材料, 研究特定人类病理中的细胞更新。 从道德上允许获取死后人体组织 被授予。 我们使用这种策略来确定成年人大脑皮层中细胞的年龄,并表明, 非神经元细胞交换,枕骨神经元与个体一样古老,支持出生后 在这个区域不发生神经发生。追溯出生日期是一种普遍适用的策略, 用于测量人体在生理和病理条件下的细胞更新。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

JONAS FRISEN其他文献

JONAS FRISEN的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('JONAS FRISEN', 18)}}的其他基金

NEURONAL TURNOVER IN ADULT BRAIN USING AMS FOR RETROSPECTIVE BIRTH DATING CELLS
使用 AMS 进行追溯出生约会细胞的成人大脑神经元周转
  • 批准号:
    8362753
  • 财政年份:
    2011
  • 资助金额:
    $ 2.85万
  • 项目类别:
NEURONAL TURNOVER IN ADULT BRAIN USING AMS FOR RETROSPECTIVE BIRTH DATING CELLS
使用 AMS 进行追溯出生约会细胞的成人大脑神经元周转
  • 批准号:
    8171680
  • 财政年份:
    2010
  • 资助金额:
    $ 2.85万
  • 项目类别:
NEURONAL TURNOVER IN ADULT BRAIN USING AMS FOR RETROSPECTIVE BIRTH DATING CELLS
使用 AMS 进行追溯出生约会细胞的成人大脑神经元周转
  • 批准号:
    7977073
  • 财政年份:
    2009
  • 资助金额:
    $ 2.85万
  • 项目类别:
NEURONAL TURNOVER IN ADULT BRAIN USING AMS FOR RETROSPECTIVE BIRTH DATING CELLS
使用 AMS 进行追溯出生约会细胞的成人大脑神经元周转
  • 批准号:
    7724083
  • 财政年份:
    2008
  • 资助金额:
    $ 2.85万
  • 项目类别:
NEURONAL TURNOVER IN ADULT BRAIN USING AMS FOR RETROSPECTIVE BIRTH DATING CELLS
使用 AMS 进行追溯出生约会细胞的成人大脑神经元周转
  • 批准号:
    7359002
  • 财政年份:
    2006
  • 资助金额:
    $ 2.85万
  • 项目类别:
NEURONAL TURNOVER IN ADULT BRAIN USING AMS FOR RETROSPECTIVE BIRTH DATING CELLS
使用 AMS 进行追溯出生约会细胞的成人大脑神经元周转
  • 批准号:
    7183233
  • 财政年份:
    2005
  • 资助金额:
    $ 2.85万
  • 项目类别:
NEURONAL TURNOVER IN ADULT BRAIN USING AMS CELL DATING
使用 AMS 细胞测年研究成人大脑中的神经元周转
  • 批准号:
    6975565
  • 财政年份:
    2004
  • 资助金额:
    $ 2.85万
  • 项目类别:

相似海外基金

Hormone therapy, age of menopause, previous parity, and APOE genotype affect cognition in aging humans.
激素治疗、绝经年龄、既往产次和 APOE 基因型会影响老年人的认知。
  • 批准号:
    495182
  • 财政年份:
    2023
  • 资助金额:
    $ 2.85万
  • 项目类别:
Investigating how alternative splicing processes affect cartilage biology from development to old age
研究选择性剪接过程如何影响从发育到老年的软骨生物学
  • 批准号:
    2601817
  • 财政年份:
    2021
  • 资助金额:
    $ 2.85万
  • 项目类别:
    Studentship
RAPID: Coronavirus Risk Communication: How Age and Communication Format Affect Risk Perception and Behaviors
RAPID:冠状病毒风险沟通:年龄和沟通方式如何影响风险认知和行为
  • 批准号:
    2029039
  • 财政年份:
    2020
  • 资助金额:
    $ 2.85万
  • 项目类别:
    Standard Grant
Neighborhood and Parent Variables Affect Low-Income Preschool Age Child Physical Activity
社区和家长变量影响低收入学龄前儿童的身体活动
  • 批准号:
    9888417
  • 财政年份:
    2019
  • 资助金额:
    $ 2.85万
  • 项目类别:
The affect of Age related hearing loss for cognitive function
年龄相关性听力损失对认知功能的影响
  • 批准号:
    17K11318
  • 财政年份:
    2017
  • 资助金额:
    $ 2.85万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Affect regulation and Beta Amyloid: Maturational Factors in Aging and Age-Related Pathology
影响调节和 β 淀粉样蛋白:衰老和年龄相关病理学中的成熟因素
  • 批准号:
    9320090
  • 财政年份:
    2017
  • 资助金额:
    $ 2.85万
  • 项目类别:
Affect regulation and Beta Amyloid: Maturational Factors in Aging and Age-Related Pathology
影响调节和 β 淀粉样蛋白:衰老和年龄相关病理学中的成熟因素
  • 批准号:
    10166936
  • 财政年份:
    2017
  • 资助金额:
    $ 2.85万
  • 项目类别:
Affect regulation and Beta Amyloid: Maturational Factors in Aging and Age-Related Pathology
影响调节和 β 淀粉样蛋白:衰老和年龄相关病理学中的成熟因素
  • 批准号:
    9761593
  • 财政年份:
    2017
  • 资助金额:
    $ 2.85万
  • 项目类别:
How age dependent molecular changes in T follicular helper cells affect their function
滤泡辅助 T 细胞的年龄依赖性分子变化如何影响其功能
  • 批准号:
    BB/M50306X/1
  • 财政年份:
    2014
  • 资助金额:
    $ 2.85万
  • 项目类别:
    Training Grant
Inflamm-aging: What do we know about the effect of inflammation on HIV treatment and disease as we age, and how does this affect our search for a Cure?
炎症衰老:随着年龄的增长,我们对炎症对艾滋病毒治疗和疾病的影响了解多少?这对我们寻找治愈方法有何影响?
  • 批准号:
    288272
  • 财政年份:
    2013
  • 资助金额:
    $ 2.85万
  • 项目类别:
    Miscellaneous Programs
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了