PROJECT 3 : QUANTITATIVE BIOMARKERS OF DNA AND PROTEIN OXIDATION

项目 3：DNA 和蛋白质氧化的定量生物标志物

• 批准号: 7602401
• 负责人: Paul Thomas Henderson
• 金额: $ 27.34万
• 依托单位: UNIVERSITY OF CALIF-LAWRNC LVRMR NAT LAB
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602401
• 关键词: Amino Acids; Animals; Arteriosclerosis; Biological; Biological Markers; Cells; Computer Retrieval of Information on Scientific Projects Database; DNA; DNA biosynthesis; DNA chemical synthesis; Diabetes Mellitus; Disease; Dose; Funding; Goals; Grant; Institution; Isotope Labeling; Label; Leukocytes; Malignant Neoplasms; Metabolic; Methods; Modification; Nucleosides; Nucleotides; Oxidative Stress; Pathway interactions; Peroxidase; Proteins; RNA; Research; Research Personnel; Resources; Source; United States National Institutes of Health; age related; chlorination; human disease; oxidation; protein metabolite

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Project 3 is developing methods to quantify oxidative modifications in DNA, proteins, and metabolites. We will focus on introducing 14C isotope labels into biological molecules in order to characterize pathways and biomarkers of relevance to human disease. This labeling is done by either dosing cells or animals with a labeled building block such as an amino acid for incorporation into proteins or a nucleoside for synthesis of DNA. Two main objectives of Project 3 are: (1) characterization of nucleotide pool oxidation products that are incorporated into DNA and RNA and (2) chlorination of proteins by HOCl produced by myeloperoxidase during the activation of white blood cells. Completion of these objectives will enable progress towards the general goal of quantifying the metabolic flux of molecules produced as a result of oxidative stress which may be related to cancer, diabetes, arteriosclerosis and numerous aging-related diseases.

这个子项目是众多研究子项目之一