EFFECTS OF CHLOROPHYLLS ON AFLATOXINB2 BIOAVAILABILITY IN HUMAN VOLUNTEERS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这项工作的大部分目标是降低黄曲霉毒素1的风险。我们已经使用鲑鱼模型来确定叶绿素(Chl)的食品级衍生物--叶绿素(CHL)是一种对黄曲霉毒素1(AFB1)有效的阻断剂。为了追求叶绿素及其衍生物作为阻滞剂的前景，我们将比较叶绿素(CHL)和天然叶绿素(Chl)在癌症化学预防和人类中的有效性。这两种药物在体外对多种致癌物都有抗突变作用。我们的指导性假设是，氯素主要通过减少致癌物的摄取和生物利用度来保护体内。我们建议在人类身上使用14C-AFB1来测试我们的中心假设。在NCI/ng剂量范围内摄入14C-AFB1后的体液加速质谱仪(AMS)应该允许我们以足够的灵敏度和可以忽略的风险来检查CHL和CHL对黄曲霉毒素生物利用度的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Much of this work targets on AFB1 risk reduction. We have used the trout model to identify chlorophyllin (CHL), a food-grade derivative of chlorophyll (Chl), as a potent blocking agent against AFB1. To pursue the promise of chlorophylls and derivatives as blocking agents, we will compare the efficacy of chlorophyllin (CHL) and natural chlorophylls (Chl) in cancer chemoprevention and humans. Both agents exert antimutagenesis behavior in vitro against a wide range of carcinogens. Our guiding hypothesis is that chlorins protect in vivo primarily via reduction of carcinogen uptake and bioavailability. We propose to test our central hypothesis in humans using 14C-AFB1. Accelerated Mass Spectrometry (AMS) of body fluids following ingestion of 14C-AFB1 in the nCi/ng dose range should allow us to examine CHL and Chl effects on aflatoxin bioavailability with adequate sensitivity and negligible risk.