EFFECTS OF CHLOROPHYLLS ON AFLATOXINB2 BIOAVAILABILITY IN HUMAN VOLUNTEERS

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。这项工作的大部分目标是降低AFB1风险。我们使用鳟鱼模型来鉴定叶绿素（CHL），叶绿素（CHL）的食品级衍生物，作为抗AFB1的有效阻断剂。为了探索叶绿素及其衍生物作为阻断剂的前景，我们将比较叶绿素（CHL）和天然叶绿素（CHL）在癌症化学预防和人类中的作用。这两种药物在体外对多种致癌物均具有抗诱变作用。我们的指导假设是，氯在体内保护主要是通过减少致癌物质的摄取和生物利用度。我们建议在人类中使用14C-AFB1来验证我们的中心假设。在nCi/ng剂量范围内摄入14C-AFB1后的体液加速质谱（AMS）应使我们能够以足够的灵敏度和可忽略的风险检查CHL和CHL对黄曲霉毒素生物利用度的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Much of this work targets on AFB1 risk reduction. We have used the trout model to identify chlorophyllin (CHL), a food-grade derivative of chlorophyll (Chl), as a potent blocking agent against AFB1. To pursue the promise of chlorophylls and derivatives as blocking agents, we will compare the efficacy of chlorophyllin (CHL) and natural chlorophylls (Chl) in cancer chemoprevention and humans. Both agents exert antimutagenesis behavior in vitro against a wide range of carcinogens. Our guiding hypothesis is that chlorins protect in vivo primarily via reduction of carcinogen uptake and bioavailability. We propose to test our central hypothesis in humans using 14C-AFB1. Accelerated Mass Spectrometry (AMS) of body fluids following ingestion of 14C-AFB1 in the nCi/ng dose range should allow us to examine CHL and Chl effects on aflatoxin bioavailability with adequate sensitivity and negligible risk.