EFFECTS OF CHLOROPHYLLS ON AFLATOXINB2 BIOAVAILABILITY IN HUMAN VOLUNTEERS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。这项工作的大部分目标是降低AFB 1风险。我们已经使用鳟鱼模型，以确定叶绿素（CHL），叶绿素（Chl）的食品级衍生物，作为一种有效的阻断剂对黄曲霉毒素B1。为了追求叶绿素及其衍生物作为阻断剂的前景，我们将比较叶绿酸（CHL）和天然叶绿素（Chl）在癌症化学预防和人类中的功效。这两种药物在体外对广泛的致癌物发挥抗突变作用。我们的指导假设是，二氢卟酚在体内保护主要是通过减少致癌物质的吸收和生物利用度。我们建议使用14 C-AFB 1在人类中测试我们的中心假设。在nCi/ng剂量范围内摄入14 C-AFB 1后的体液加速质谱（AMS）应允许我们检查CHL和Chl对黄曲霉毒素生物利用度的影响，具有足够的灵敏度和可忽略的风险。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Much of this work targets on AFB1 risk reduction. We have used the trout model to identify chlorophyllin (CHL), a food-grade derivative of chlorophyll (Chl), as a potent blocking agent against AFB1. To pursue the promise of chlorophylls and derivatives as blocking agents, we will compare the efficacy of chlorophyllin (CHL) and natural chlorophylls (Chl) in cancer chemoprevention and humans. Both agents exert antimutagenesis behavior in vitro against a wide range of carcinogens. Our guiding hypothesis is that chlorins protect in vivo primarily via reduction of carcinogen uptake and bioavailability. We propose to test our central hypothesis in humans using 14C-AFB1. Accelerated Mass Spectrometry (AMS) of body fluids following ingestion of 14C-AFB1 in the nCi/ng dose range should allow us to examine CHL and Chl effects on aflatoxin bioavailability with adequate sensitivity and negligible risk.