DEVELOPMENTAL NEUROBIOLOGY IMAGING AND TISSUE PROCESSING CORE

核心C 开发神经生物学成像和组织处理核心(核心C)提供最先进的 为突触组装和调制的实验项目提供设备和技术支持， 神经元和神经胶质的结构和功能。通过共享技术专长、设备、设施和 作为专业的员工，核心促进了具有成本效益的跨项目协作。发展中的 神经生物学成像和组织处理核心执行细胞学和组织学处理 实验组织，并以规则和可靠的方式进行图像分析，为发育 突触神经生物学、神经免疫内分泌学和传染病，以及分子生物学和 在MRRC中概述的基因研究项目。特别是，发育性神经生物学成像和 组织处理核心提供用于细胞和组织处理以及定量图像分析的设施， 组成MRRC产品组合的许多项目的光、共焦和电子显微镜水平。在这 在这方面，成像核心有助于各组成部分项目之间的相互关系和协同作用， 从而产生比单个项目更高的科学生产率和更高的成本效益 分开实现。目前重新配置的神经生物学组织处理和成像核心是 合并两个先前存在的核心(发育神经生物学和组织处理以及 结合了LSCM成像和电生理学)，从原始的具有两个清晰描绘的目标 P30。对于许多研究人员来说，这两个目标是相继的，因此导致了相继 使用两个互不重叠的设施。现在，这些任务被整合在一起，使调查人员能够 完成细胞或组织处理和各种成像方式，包括：延时实时成像 稳定、长期的高分辨率形态研究；同时实时双光子激光扫描 共聚焦显微镜全细胞膜片钳对质膜电位的成像与记录 技术；通过脱染率监测单个突触的突触前释放概率 FM荧光染料.包括重建的连续切片分析的高分辨率电子显微镜 细胞；免疫组织化学染色和电子显微镜相结合的亚细胞定位 一个单位的表位。这些技术需要调查人员不断提高技能。 以及在仪器设备方面的重大投资。

Core C The Developmental Neurobiology Imaging and Tissue Processing Core (Core C) provides state-of-the-art equipment and technical support for experimental projects on the assembly and modulation of synaptic, neuronal, and glial structure and function. By sharing technical expertise, equipment, facilities, and professional staff, the Core facilitates cost-effective, cross-project collaborations. The Developmental Neurobiology Imaging and Tissue Processing Core performs cytological and histological processing of experimental tissues, and performs image analysis in a regular and reliable fashion for the developmental synaptic neurobiology, neuroimmunoendocrinology and infectious disease, and molecular biological and genetic studies projects outlined in the MRRC. In particular, the Developmental Neurobiology Imaging and Tissue Processing Core provides a facility for cell and tissue processing and quantitative image analysis at the light, confocal, and electron microscopic levels for many projects comprising the MRRC portfolio. In this regard, the Imaging Core contributes to an interrelationship and synergism among the component projects, resulting in greater scientific productivity and improved cost effectiveness than individual projects could achieve separately. The currently re-configured Neurobiology Tissue Processing and Imaging Core is the result of a merger of two previously existing cores (developmental neurobiology and tissue processing AND combined LSCM imaging and electrophysiology) that had two clearly delineated objectives from the original P30. For many investigators, these two objectives are sequential and therefore have led to a sequential use of two non-overlapping facilities. Now, these tasks are integrated allowing an investigator to accomplish cell or tissue processing and various imaging modalities including: time-lapse live imaging for stable, long-term high-resolution morphological studies; simultaneous real time two photon laser scanning confocal microscopy imaging and recording of plasma membrane potential by the whole-cell patch-clamp technique; monitoring of presynaptic release probability of individual synapses by the de-staining rate of the FM fluorescent dyes; high resolution electron microscopy including serial section analysis of reconstructed cells; and combined immunohistochemical staining and electron microscopy for subcellular localization of epitopes a single unit. These techniques require constantly evolving skills on the side of the investigators and a significant investment in instrumentation.