DEVELOPMENTAL NEUROBIOLOGY IMAGING AND TISSUE PROCESSING CORE

发育神经生物学成像和组织处理核心

• 批准号: 7563388
• 负责人: Lucas D Pozzo-Miller
• 金额: $ 23.82万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7563388
• 关键词: Adoption; Argon; Arts; Biological; Cell membrane; Cells; Chromosome Pairing; Collaborations; Communicable Diseases; Complement 3a; Core Facility; Cultured Cells; Data Analyses; Development; Developmental Disabilities; Electron Microscopy; Electron Microscopy Facility; Electrons; Electrophysiology (science); Epitopes; Equipment; Experimental Designs; Faculty; Fluorescent Dyes; Funding; Genetic; Human Resources; Image; Image Analysis; Imaging technology; Immersion Investigative Technique; Immunoelectron Microscopy; Individual; Investments; Knowledge; Krypton; Label; Laser Scanning Confocal Microscopy; Laser Scanning Microscopy; Lasers; Learning; Life; Light; Logic; Membrane Potentials; Mental Retardation; Microscope; Microscopic; Microscopy; Mind; Mission; Modality; Molecular; Monitor; Neonatal; Nervous system structure; Neurobiology; Neurons; Numbers; Patch-Clamp Techniques; Physiologic pulse; Positioning Attribute; Probability; Procedures; Process; Productivity; Pulse taking; Quantitative Microscopy; Range; Rate; Research; Research Personnel; Resolution; Running; Scanning; Services; Side; Slice; Staining method; Stains; Standards of Weights and Measures; Stretching; Structure; Synapses; System; Technical Expertise; Techniques; Time; Tissues; Training; Transfection; Water; caN protocol; comparative; cost; cost effective; cost effectiveness; data acquisition; developmental neurobiology; experience; fetus cell; improved; insight; instrument; instrumentation; interest; intracellular protein transport; mature animal; member; neglect; patch clamp; presynaptic; protein localization location; protein transport; ranpirnase; research study; sample fixation; skills; success; synergism; tissue fixing; tissue processing; two-photon

Core C The Developmental Neurobiology Imaging and Tissue Processing Core (Core C) provides state-of-the-art equipment and technical support for experimental projects on the assembly and modulation of synaptic, neuronal, and glial structure and function. By sharing technical expertise, equipment, facilities, and professional staff, the Core facilitates cost-effective, cross-project collaborations. The Developmental Neurobiology Imaging and Tissue Processing Core performs cytological and histological processing of experimental tissues, and performs image analysis in a regular and reliable fashion for the developmental synaptic neurobiology, neuroimmunoendocrinology and infectious disease, and molecular biological and genetic studies projects outlined in the MRRC. In particular, the Developmental Neurobiology Imaging and Tissue Processing Core provides a facility for cell and tissue processing and quantitative image analysis at the light, confocal, and electron microscopic levels for many projects comprising the MRRC portfolio. In this regard, the Imaging Core contributes to an interrelationship and synergism among the component projects, resulting in greater scientific productivity and improved cost effectiveness than individual projects could achieve separately. The currently re-configured Neurobiology Tissue Processing and Imaging Core is the result of a merger of two previously existing cores (developmental neurobiology and tissue processing AND combined LSCM imaging and electrophysiology) that had two clearly delineated objectives from the original P30. For many investigators, these two objectives are sequential and therefore have led to a sequential use of two non-overlapping facilities. Now, these tasks are integrated allowing an investigator to accomplish cell or tissue processing and various imaging modalities including: time-lapse live imaging for stable, long-term high-resolution morphological studies; simultaneous real time two photon laser scanning confocal microscopy imaging and recording of plasma membrane potential by the whole-cell patch-clamp technique; monitoring of presynaptic release probability of individual synapses by the de-staining rate of the FM fluorescent dyes; high resolution electron microscopy including serial section analysis of reconstructed cells; and combined immunohistochemical staining and electron microscopy for subcellular localization of epitopes a single unit. These techniques require constantly evolving skills on the side of the investigators and a significant investment in instrumentation.

芯C 发育神经生物学成像和组织处理核心（核心C）提供了最先进的 为突触组装和调节实验项目提供设备和技术支持， 神经元和神经胶质的结构和功能。通过分享技术专长、设备、设施， 核心小组拥有专业的工作人员，可促进具有成本效益的跨项目协作。发育 神经生物学成像和组织处理核心执行细胞学和组织学处理， 实验组织，并进行图像分析，在一个定期和可靠的方式为发展 突触神经生物学、神经免疫内分泌学和感染性疾病、分子生物学和 MRRC中概述的遗传研究项目。特别是，发育神经生物学成像和 Tissue Processing Core提供细胞和组织处理以及定量图像分析的设施， 光，共聚焦，和电子显微镜水平的许多项目，包括MRRC的投资组合。在这 在这方面，成像核心有助于各组成项目之间的相互关系和协同作用， 从而提高科学生产力和成本效益，而不是单个项目 分别实现。目前重新配置的神经生物学组织处理和成像核心是 这是两个先前存在的核心（发育神经生物学和组织处理， 结合LSCM成像和电生理学），具有两个清晰描绘的目标， P30。对于许多研究者来说，这两个目标是连续的，因此导致了一个连续的过程。 使用两个不重叠的设施。现在，这些任务被整合在一起， 完成细胞或组织处理和各种成像模式，包括： 稳定的、长期的高分辨率形态学研究;同时进行的真实的时间双光子激光扫描 共聚焦显微成像和全细胞膜片钳记录质膜电位 技术;监测突触前释放概率的个别突触的脱色率 FM荧光染料;高分辨电子显微术，包括重建的连续切片分析 细胞;并结合免疫组化染色和电子显微镜进行亚细胞定位， 表位为一个单位。这些技术要求调查人员不断提高技能 以及对仪器设备的大量投资。