CORE--CELLULAR NEUROSCIENCE CORE

最初（1999年）的申请不包括细胞神经科学核心。相反，它提出了单独的核心， 神经病理学和神经科学，后者提供细胞培养方面的专业知识。的主任 神经科学核心是大卫快乐，医学博士，他是一位国际公认的神经学家， 外周和中枢神经系统生物学。神经病理学最初是由约翰 Trojanowski，医学博士，博士和医学博士露西·洛克杰出的神经病理学家 神经退行性疾病和小儿脑肿瘤。随着用户需求的发展，很明显， 这些设施应该合并成一个单一的细胞神经科学核心。这是在1994年与博士。 作为导演的乐趣他担任这一职务，通过更新的中心（1999年），直到2001年，当 领导权交给了杰弗里·戈尔登博士，他是一位儿科神经病理学家，主要研究大脑 发展及其障碍。Golden博士于1997年首次加入CHOP， MRDDRC，作为用户和细胞神经科学核心的副主任。快乐博士 继续担任CHOP斯托克斯研究所所长。 自成立以来（1994年），我们的核心为用户提供了一个国家的最先进的方法， 可视化基因产物在正常的、发育中的神经细胞和那些经历了 各种形式的退化和再生。我们不断地急切地增加新的技能， 仪器和试剂，以更好地满足用户的需求。1995年，在联合国的慷慨援助下， 儿童医院，我们购买了徕卡共聚焦显微镜，我们添加了倒置显微镜，分期安装 显微操作器/显微注射器，以及安装在工作台上的环境室，从而允许 在生理条件下长时间观察和操作活细胞。以这种方式，我们 提供8色荧光成像。我们的机构支付了仪器的费用， 资金用于部分支持与共聚焦主任Peter Bannerman博士合作的技术人员 显微镜班纳曼博士在使用这种仪器方面变得非常熟练， 向MRDDRC研究人员提供专业知识。 我们对核心库进行了其他几项值得注意的技术补充，包括原位杂交， 切片和整个胚胎。我们的总体目标不仅是提供一种技术， 咨询服务，促进方法的实施以及数据的解释。我们 为了更好地支持MRDDRC，我们还增加了视频增强显微镜， 研究人员在分析细胞内Ca ~（2+）和Na ~+以及估计线粒体电位。 我们特别高兴的是，道格拉斯库尔特博士在1999年加入CHOP后加入了核心。博士 库尔特增加了我们的“医疗设备”，他在电生理学和他的背景， 研究癫痫我们还欢迎（2003年）亚历克斯·贾金斯博士，一位儿科神经病理学家， 小儿脑损伤和小儿脑肿瘤与非典型畸胎样/横纹肌样肿瘤和原发性 尤其是神经外胚层肿瘤在病理学系和CHOP基金的支持下，Dr. Judkins监督新装备的组织病理学实验室组织微阵列仪和激光捕获显微镜 系统.这些系统目前正由中心调查人员使用， 由分子遗传学核心和分析神经化学核心提供。 遗传学的进步迅速加速了与糖尿病发病机制有关的基因的发现。 发育障碍，包括我们的MRDDRC成员已经确定的几个基因。到 为了更好地理解基因型-表型关系，细胞神经科学核心计划提供转基因 和诱变服务。目标是为特定的 目的是研究与MRDDRC使命相关的疾病。戈尔登博士负责建立了一个 在CHOP执行这些服务。MRDDRC计划提供咨询和经济的访问， 新设施。这一令人兴奋的进展将有助于研究人员仔细研究迄今为止的疾病基础。 无法触及的细节事实上，我们满意地注意到，MRDDRC用户不仅将探索这些模式， 与我们的核心提供的技术，但与过多的方法，可通过 分子遗传学、分析神经化学和光谱学核心。 由于这一核心的不断发展以及该机构对新技术和新技术的投资， 设备的不断升级，细胞神经科学核心已经能够提供出色的 为中心用户提供服务。在过去的4.5年里，我们为25名研究人员提供了服务;为至少49名研究人员提供了服务。 出版物。现有服务的增长和新方法的增加确保了MRDDRC成员 这些技术对所有当代跨学科研究都是必不可少的 research.

The initial (1999) application did not include a Cellular Neuroscience Core. Instead, it proposed separate cores in Neuropathology and Neuroscience, with the latter offering expertise in cell culture. The Director of the Neuroscience Core was David Pleasure, M.D., an internationally recognized neurologist with interests in glial biology of the peripheral and central nervous system. Neuropathology initially was headed by John Trojanowski, M.D., Ph.D. and Dr. Lucy Rorke, M.D., distinguished neuropathologists with respective eminence in neurodegenerative diseases and pediatric brain tumors. As user needs evolved, it became apparent that these facilities should be merged into a single Cellular Neuroscience Core. This was accomplished in 1994 with Dr. Pleasure as the Director. He served in this capacity through the renewal of the Center (1999) until 2001, when leadership passed to Dr. Jeffrey Golden, a pediatric neuropathologist with a primary interest in brain development and its disorders. Dr. Golden first joined CHOP in 1997 and immediately became engaged with the MRDDRC, both as a user and as an Associate Director of the Cellular Neuroscience Core. Dr. Pleasure has continued to serve as the Director of the Stokes Research Institute at CHOP. Since its inception (1994) our Core has provided users with a diverse repertoire of state-of-the-art methods for visualization of the distributions of gene products in normal, developing neural cells and in those undergoing various forms of degeneration and regeneration. We have continually and eagerly added new skills, instrumentation and reagents to better serve our users's needs. In 1995, with the generous assistance of the Children's Hospital, we purchased a Leica confocal microscope to which we added inverted microscopy, stagemounted micromanipulators/microinjectors, and a stage-mounted environmental chamber, thus permitting prolonged observation and manipulation of living cells under physiological conditions. In this manner we offered 8-color capacity fluorescent imaging. Our institution paid for the apparatus and we used MRDDRC funding to partially support a technician who worked with Dr. Peter Bannerman, the Director of Confocal Microscopy. Dr. Bannerman became very skillful in the use of this instrument and he made this valuable expertise available to MRDDRC researchers. We made several other notable technological additions to the Core repertoire, including in situ hybridization in both sections and whole embryos. Our general purpose has been not only to make available a technology, but a consultative service that facilitates implementation of the method as well as the interpretation of data. We adhered to this policy when we also added video-enhanced microscopy in order to better support MRDDRC investigators in analyzing intracellular Ca2+ and Na+ as well as the estimate of mitochondrial potential. We were especially gratified that Dr. Douglas Coulter joined the Core upon his coming to CHOP in 1999. Dr. Coulter added to our "armamentarium" his deep experience in electrophysiology and his background in studying the epilepsies. We also welcomed (2003) Dr. Alex Judkins, a pediatric neuropathologist with interests in pediatric brain injury and pediatric brain tumors v atypical teratoid/rhabadoid tumors and primative neuroectodermal tumors in particular. Supported by funds from the Department of Pathology and CHOP, Dr. Judkins oversees the newly equipped histopathology labs tissue microarrayer and laser-capture microscopy systems. These systems, which are now being utilized by center investigators, will also complement services provided by the Molecular Genetics core and the Analytical Neurochemistry cores. Advances in genetics have rapidly accelerated the discovery of genes implicated in the pathogenesis of the developmental disabilities, including several genes that have been identified by members of our MRDDRC. To better appreciate genotype-phenotype relationships, the Cellular Neuroscience Core plans to offer transgenic and mutagenesis services during the next funding period. The goal is to generate animal models for the specific intent to study disorders relevant to the MRDDRC mission. Dr. Golden has overseen the establishment of a core at CHOP to perform these services. The MRDDRC plans to provide consultation and economical access to this new facility. This exciting advance will help investigators to scrutinize the basis of disease in hitherto inaccessible detail. Indeed, we note with satisfaction that MRDDRC users will explore these models not only with the techniques that our Core offers, but with the plethora of methods that are available through the Molecular Genetics and Analytical Neurochemistry and Spectroscopy Cores. As a result of the continued evolution of this core and the investment by the institution in new technologies and the constant upgrading of equipment, the Cellular Neuroscience Core has been able to provide outstanding services to Center users. Over the past 4.5 years we have serviced 25 investigators; contributing to at least 49 publications. The growth of existing services and the addition of new methods ensures that MRDDRC members enjoy ready access to that technological array that has become so essential to all contemporary interdisciplinary research.