EVALUATION OF NORMALS/ BRONCHOPULMONARY LUNG LAVAGE/ BRONCHIAL BRUSHING ETC

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 本研究的目的是从正常人的血液和肺中获取生物材料，以建立一套各种参数的正常范围。这将为各种肺部疾病的个体应用提供重要信息，并有助于了解各种肺部疾病的病因和发病机制。 (A)从支气管肺泡灌洗(BAL)中获得的巨噬细胞将被用来评估使用不同腺病毒载体进行基因转移的效率；(B)将进行研究，以评估减少不同HIV毒株感染巨噬细胞的策略；(C)将根据腺病毒载体感染巨噬细胞后释放细胞因子的模式进行评估；(D)将对(A)和(C)中所述的实验进行类似的实验，通过刷洗肺部呼吸道收集呼吸道上皮细胞；(E)将对BAL收集的肺上皮衬里液进行抗腺病毒抗体、各种细胞因子和血管内皮生长因子水平的评估； (F)将使用微阵列对收集的呼吸道细胞进行各种基因表达研究，以了解吸烟对肺部呼吸道细胞(呼吸道上皮细胞)中抗氧化剂和其他基因表达的影响，以及这些变化与肺癌相关基因突变变化的频率之间的关系。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this study is to obtain biologic materials from the blood and lungs of normal individuals to establish a set of normal range for various parameters. These will provide important information when applied to individuals with various pulmonary diseases, and will help in understanding of the etiology and pathogenesis of various lung diseases. Samples obtained from fiberoptic bronchoscopy in normal individuals will be used as follows: (a) macrophages obtained from bronchoalveolar lavage (BAL) will be used to evaluate efficiency of gene transfer using different adenovirus vectors; (b) studies will be conducted to evaluate strategies to decrease infection of macrophages by different strains of HIV; (c) macrophages will be evaluated in terms of pattern of cytokine release following infection with adenovirus vectors; (d) similar experiments as the ones described in (a) and (c) will be conducted with airway epithelial cells collected by brushing the lung airways; (e) lung epithelial lining fluid collected by BAL will be evaluated for anti-adenovirus antibodies, for various cytokines and for vascular endothelial growth factor levels; (f) collected airway cells will be evaluated for the various gene expression studies using microarrays for the effects of smoking in the expression of antioxidant and other genes in the cells lining the airways of the lungs (airway epithelial cells) and how these changes correlate with the frequency in mutation changes of genes that are relevant to lung cancer.