P5: DIRECTED STEM CELL DIFFERENTIATION - HUMAN APPLICATION

P5：定向干细胞分化 - 人体应用

• 批准号: 7610574
• 负责人: GERALD ALEXANDER COLVIN
• 金额: $ 11.44万
• 依托单位: ROGER WILLIAMS HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7610574
• 关键词: Activities of Daily Living; Bone Marrow Transplantation; Cell Cycle; Cell Differentiation process; Cell Transplants; Cells; Computer Retrieval of Information on Scientific Projects Database; Development; Funding; Grant; Growth; Human; Institution; Lead; Marrow; Modeling; Mus; Outcome; Patients; Population; Property; Research; Research Personnel; Resources; Signal Transduction; Source; Stem cells; Techniques; Testing; Transplantation; United States National Institutes of Health; Work; base; bioprocess; cytokine; human stem cells; improved; in vivo; in vivo Model; mortality; programs; research study; stem

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. There are two specific aims to this project. Aim one involves human cell stem/progenitor cells. We will be performing parallel experiments based on our murine work. We have found that it is possible to "program" the stem/progenitor cells to make specific progeny by giving them a signal. This signal is composed of cytokines. We have found that at certain points in cell cycle, the stem/progenitor cells are more sensitive and open to receive these signals. What makes this unique is that we are able to direct these cells before they begin dividing, which means that we do not take away any of their full growth potential or stem cell properties. It is hoped that with more work, we are able to "program" these cells to make a specific lineage and transplant these cells into a patient that is deficient in that specific lineage. The potential application is broad and this work could end up changing the way we perform bone marrow transplantation. In addition, this work has the potential to make bone marrow transplantation safer with improved survival as some transplants we perform still has an upfront mortality approaching 50%. Aim two involves using the results of aim one and testing the "programmed" cell population in an in vivo model. We will determine the in vivo functional capacity of directed human marrow populations derived from aim one with a transplant model. Overall, the results from this project will be crucial to the development of successful bioprocessing techniques. The techniques can eventually lead to improvement in bone marrow transplantation outcomes.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 该项目有两个具体目标。目标一涉及人类细胞干/祖细胞。我们将根据我们的小鼠工作进行平行实验。我们发现可以通过向干细胞/祖细胞发出信号来“编程”它们以产生特定的后代。该信号由细胞因子组成。我们发现，在细胞周期的某些点，干细胞/祖细胞更加敏感并且开放地接收这些信号。其独特之处在于我们能够在这些细胞开始分裂之前对其进行引导，这意味着我们不会剥夺它们的任何完整生长潜力或干细胞特性。希望通过更多的工作，我们能够对这些细胞进行“编程”，使其形成特定的谱系，并将这些细胞移植到缺乏该特定谱系的患者体内。潜在的应用很广泛，这项工作最终可能会改变我们进行骨髓移植的方式。此外，这项工作有可能使骨髓移植更安全并提高生存率，因为我们进行的一些移植的前期死亡率仍然接近 50%。目标二涉及使用目标一的结果并在体内模型中测试“编程”细胞群。 我们将通过移植模型确定源自目标一的定向人类骨髓群的体内功能能力。 总体而言，该项目的结果对于成功开发生物加工技术至关重要。这些技术最终可以改善骨髓移植的结果。