Ultrasound Technologies for Tissue Engineering

组织工程超声技术

基本信息

批准号：
7632281
负责人：
DIANE DALECKI
金额：
$ 43.48万
依托单位：
UNIVERSITY OF ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-09-24 至 2011-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7632281
关键词：
3-Dimensional Acoustics Active Sites Affect Allogenic Autologous Biochemical Biocompatible Materials Biological Biological Monitoring Biology Biomechanics Bioreactors Cell Count Cell Proliferation Cell physiology Cells Clinical Treatment Collagen Collagen Fibril Complex Cultured Cells DNA Data Deposition Dermal Development Engineering Environment Epithelial Cells Exposure to Extracellular Matrix Extracellular Matrix Proteins Fibroblasts Fibronectins Frequencies Gel Goals Growth Healed Heart Valves Histocompatibility Testing Image Implant In Situ In Vitro Knowledge Mechanical Stress Mechanics Mediating Medical Medicine Monitor Natural regeneration Organ Patients Physiologic pulse Polymers Process Property Proteins Pulsatile Flow Radiation Regenerative Medicine Role Science Signal Transduction Skin Stress Structure Techniques Technology Tensile Strength Testing Therapeutic Uses Thick Time Tissue Engineering Tissues Translations Ultrasonic Therapy Ultrasonography Vascular Graft Wound Healing abstracting analog base bone healing cell behavior cell growth cell motility clinical practice conditioning cost design experience extracellular healing implantation in vivo innovation interdisciplinary approach mechanical drive meetings migration new technology novel strategies polymerization pressure repaired scaffold soft tissue success tissue regeneration tool ultrasound biological effect wound

项目摘要

DESCRIPTION (provided by applicant): The successful construction of engineered tissues requires the re-creation of a biologically active extracellular environment in which to develop the product. Ultrasound therapy is currently used clinically to promote bone healing and has been shown to enhance soft tissue repair. Some biological effects of ultrasound are known to result from the mechanical forces associated with acoustic wave propagation. In vitro studies demonstrate that mechanical stresses positively affect both extracellular matrix (ECM) organization and cell behavior. Thus, we hypothesize that acoustically-driven mechanical forces can be used to control ECM deposition and promote cell and tissue function. In this proposal, we combine our knowledge of ECM biology and biomedical ultrasound to develop ultrasound-based enabling technologies for the fabrication and monitoring of functional 3D ECM and tissue analogs. To accomplish this goal, we have developed four specific aims. In Aim 1, we will use ultrasound fields to fabricate ECM FN and collagen analogs, and optimize ultrasound exposure conditions that enhance ECM-mediated fibroblast and epithelial cell growth. In Aim 2, we will develop the use of acoustically-driven mechanical forces to promote fibroblast migration into 3D, collagen- based tissue constructs. In Aim 3, we will use acoustic fields to stimulate ECM organization in order to engineer the biological and material properties of collagen-based tissue constructs. In Aim 4, we will apply and extend our experience in ultrasound tissue characterization technologies to non-destructively quantify mechanical and biological properties of engineered tissues in real-time. We envision immense potential for the use of ultrasound technologies to provide break-through technologies for tissue fabrication and monitoring. Furthermore, the ability of ultrasound to propagate through tissue as a focused beam has the potential to provide a revolutionary approach to locally regulate and monitor tissue regeneration deep within the body. Tissue engineering is a potentially revolutionary approach for replacing or regenerating diseased or destroyed organs and tissues. The current lack of available tissue analogs reflects an inability to create 3-D scaffolds that have both biological activity and adequate mechanical strength. We will develop ultrasound- based enabling technologies with the dual capacity to (i) create biologically-active, 3-D tissue constructs for tissue engineering and (ii) non-invasively monitor the biological and mechanical properties of these constructs. (End of Abstract)

描述（由申请人提供）：工程组织的成功构造需要重新创建生物活跃的细胞外环境，以开发产品。目前在临床上使用超声治疗来促进骨骼愈合，并已显示可增强软组织修复。已知超声的某些生物学作用是由与声波传播相关的机械力引起的。体外研究表明，机械应力对细胞外基质（ECM）组织和细胞行为均积极影响。因此，我们假设声学驱动的机械力可用于控制ECM沉积并促进细胞和组织功能。在此提案中，我们结合了对ECM生物学和生物医学超声的了解，以开发基于超声的促成技术，用于制造和监测功能性3D ECM和组织类似物。为了实现这一目标，我们开发了四个特定的目标。在AIM 1中，我们将使用超声场来制造ECM FN和胶原蛋白类似物，并优化超声暴露条件，从而增强ECM介导的成纤维细胞和上皮细胞的生长。在AIM 2中，我们将开发使用声学的机械力来促进成纤维细胞迁移到3D，基于胶原蛋白的组织构建体。在AIM 3中，我们将使用声场来刺激ECM组织，以设计基于胶原蛋白的组织结构的生物学和材料特性。在AIM 4中，我们将在超声组织表征技术中应用和扩展我们的经验，以实时量化工程组织的机械和生物学特性。我们设想使用超声技术为组织制造和监测提供突破性技术的巨大潜力。此外，超声通过组织作为聚焦束传播的能力具有提供革命性的方法来局部调节和监测体内深处的组织再生。组织工程是替代或再生患病或破坏器官和组织的潜在革命性方法。目前缺乏可用的组织类似物反映出无法创建具有生物活性和足够机械强度的3-D支架。我们将开发具有双重能力的超声基于（i）为组织工程创建生物学活性的3D组织构建体的功能，以及（ii）非侵入性监测这些构建体的生物学和机械性能。（抽象的结尾）