Alkali production in human dental plaque and saliva as predictor of caries risk

人类牙菌斑和唾液中的碱生成可作为龋齿风险的预测因子

• 批准号: 7581196
• 负责人: Valeria V Gordan
• 金额: $ 21.98万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-30 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7581196
• 关键词: Acids; Address; Adult; Affect; Age; Alkalies; Alkalinization; Ammonia; Arginine; Arginine deiminase; Bacteria; Behavioral; Biological; Carbohydrates; Carbon Dioxide; Caries prevention; Case-Control Studies; Catabolism; Classification; Clinical; Clinical Research; Cohort Studies; Consumption; Cytoplasm; Data; Dental; Dental Enamel; Dental Plaque; Dental caries; Development; Disease; Early identification; Ecology; Environment; Enzymes; Epidemiologic Studies; Fermentation; Frequencies; Gender; Generations; Goals; Grant; Growth; Health; Human; Hydrolysis; In Vitro; Incidence; Individual; Knowledge; Lead; Measures; Methods; Numbers; Oral; Oral health; Organism; Patients; Polymerase Chain Reaction; Predisposition; Prevention; Prevention program; Prevention strategy; Probability; Production; Public Health; Race; Recording of previous events; Research; Resistance; Risk; Risk Assessment; Risk Factors; Role; Saliva; Salivary; Sampling; Severities; Societies; Source; Streptococcus gordonii; Streptococcus parasanguis; Streptococcus salivarius; Streptococcus sanguis; Time; Tooth structure; Urea; Urease; age group; base; demineralization; design; enzyme activity; experience; health disparity; human subject; in vivo; information gathering; killings; microbial; novel; novel strategies; oral bacteria; oral biofilm; oral care; oral streptococci; prevent; prospective; remineralization; research study; social

DESCRIPTION (provided by applicant): Caries continues to be a prevalent disease with high incidence among all age groups. Ammonia production from the hydrolysis of arginine and urea by enzymes of oral bacteria moderates plaque acidification and may inhibit dental caries, as suggested by in vitro studies and indirect clinical observations. The objective of this study is to examine the relationship of arginine deiminase (AD) and urease activities with dental caries status at the clinical level. We hypothesize that AD and urease activities in plaque and/or saliva of caries free subjects is significantly higher than in caries active subjects. AD and urease activities will be measured in dental plaque and saliva samples from caries free subjects, caries experienced subjects, and subjects with at least 4 teeth with active caries. Specific Aim (SA) 1 will determine the magnitude and the direction of the association between plaque and saliva AD and urease activity levels with dental caries in human subjects. SA 2 will examine the relationship between AD and urease activity levels with the numbers of selected major acid-producing and alkali-producing bacterial species in the oral samples. SA 3 will investigate the predictors of AD and urease activity levels. SA 1 will be met through a case-control study and SA 2 will be met using a sub-sample of the subjects (10 with the highest, 10 with the lowest, and 10 with medium plaque AD and urease levels). SA3 will be met by gathering information on patient's age, gender, race, dental history, plaque, and frequency of oral care, and investigating the relationship of these explanatory factors to AD and urease activity levels in plaque and saliva. If the results of the present study support an association between AD and/or urease activity levels with dental caries in adults, the knowledge obtained will be applied to the design of a prospective cohort study at the end of the grant period, which will determine whether this association is a cause-and-effect one. If AD and urease activity levels are shown to be predictive of caries risk in adults, either alone or in combination with other risk factors, we will then apply this relationship to the development of novel approaches for early identification of individuals at risk to develop caries and for caries prevention strategies. PUBLIC HEALTH RELEVANCE: The proposed study will contribute to the development of novel methods for caries risk assessment and prevention, while providing a better understanding of unknown factors affecting caries risk.

描述（由申请人提供）：龋齿仍然是一种流行病，在所有年龄组中发病率都很高。体外研究和间接临床观察表明，口腔细菌酶水解精氨酸和尿素产生的氨可减缓牙菌斑酸化，并可能抑制龋齿。本研究的目的是探讨精氨酸脱亚胺酶（AD）和尿素酶活性与龋病的关系。我们假设，AD和尿素酶活性的菌斑和/或唾液中的无龋受试者显着高于龋齿活跃的主题。AD和尿素酶活性将在来自无龋齿受试者、有龋齿受试者和至少有4颗活动性龋齿牙齿的受试者的牙菌斑和唾液样品中测量。特定目标（SA）1将确定人类受试者中牙菌斑和唾液AD以及脲酶活性水平与龋齿之间的关联的大小和方向。SA 2将检查AD和尿素酶活性水平与口腔样本中选定的主要产酸和产碱细菌物种数量之间的关系。SA 3将研究AD和尿素酶活性水平的预测因子。SA 1将通过病例对照研究来满足，SA 2将使用受试者的子样本（10个具有最高斑块AD和尿素酶水平，10个具有最低斑块AD和尿素酶水平，10个具有中等斑块AD和尿素酶水平）来满足。通过收集患者年龄、性别、种族、牙科病史、牙菌斑和口腔护理频率的信息，并研究这些解释因素与AD和牙菌斑和唾液中尿素酶活性水平的关系，来满足SA3。如果本研究的结果支持AD和/或尿素酶活性水平与成人龋齿之间的关联，则所获得的知识将应用于资助期结束时的前瞻性队列研究的设计，这将确定这种关联是否是因果关系。如果AD和尿素酶活性水平被证明是预测龋齿的风险在成年人中，无论是单独或与其他危险因素相结合，我们将应用这种关系的发展新的方法，早期识别个人的风险发展龋齿和龋齿预防策略。 公共卫生相关性：这项研究将有助于发展新的方法，龋齿风险评估和预防，同时提供了一个更好的了解未知因素影响龋齿的风险。