Imaging Core

成像核心的功能是测试新的成像方法和图像分析算法， 简化这些用于活体皮质可塑性成像的技术，并提供优化的方案和 将软件提供给中心的调查人员。成像核心优化的方法将用于 多学科综合研究中心的项目1、2和3 解剖上的皮质可塑性。 核心是一种经济高效的方式，为调查人员提供成像和分析技术 中心。具体地说，核心将： 1.筛选新型转基因小鼠，寻找标记神经特定方面的体内成像标记 解剖学，如特定的抑制性中间神经元和板层特定的锥体神经元。 2.记录哪些标记线足够亮，可用于活体成像，以及这种亮度如何随 发展。 3.标记线在视觉皮质、桶状皮质和前扣带回皮质中表达的目录 4.筛选最新的神经功能遗传编码标记物(如钙指示剂和 电压传感器)，以确定哪些适合活体成像。 5.开发新的图像分析工具，自动对轴突和树突结构进行量化 可塑性(例如，分支、分支长度、脊柱体积、脊柱和脊椎密度、脊柱动力学的变化)。 6.不断调整手术准备，以便对皮质神经元进行实时成像。 通过将这些任务分配给一个设施，中心获得了规模经济，从而将时间投入到 解决这些重要问题的工作一旦被核心所吸收。因此，中心的每个实验室都不是 个人承担着完成和优化工作所需的时间、人力和资源成本 完成中心提出的项目所需的成像方法。此外， 核心将确保每个实验室使用的成像方法得到每个实验室的一致同意 该中心的成员，并具有尽可能最高的质量。

The function of the Imaging Core is to test novel imaging approaches and image analysis algorithms, streamline these techniques for in vivo imaging of cortical plasticity, and supply the optimized protocols and software to the investigators in the Center. The approaches optimized by the Imaging Core will be used in Projects 1, 2 and 3 of the Center for multidisciplinary, comprehensive studies of the role of specific genes in anatomical cortical plasticity. The Core is a cost effective and efficient way of providing imaging and analysis techniques to investigators in the Center. Specifically, the Core will: 1. Screen novel transgenic mice for markers for in vivo imaging that label specific aspects of neural anatomy, such as specific inhibitory interneurons and laminar-specific pyramidal neurons. 2. Catalog which labeled lines are bright enough for in vivo imaging and how this brightness changes with development. 3. Catalog which labeled lines express in visual, barrel, and anterior cingulate cortices 4. Screen newly-advanced genetically-encoded markers of neuronal function (e.g. calcium indicators and voltage sensors) to determine which are suitable for in vivo imaging. 5. Develop novel image analysis tools to automate the quantification of axonal and dendritic structural plasticity (e.g. changes in branching, branch length, spine volume, spine and bouton density, spine kinetics). 6. Continually fine-tune the surgical preparation for live imaging of cortical neurons. By assigning these tasks to one facility, the Center gains an economy of scale in which the time devoted to working through these important issues is absorbed once by the Core. Thus, each lab in the Center is not individually burdened with the costs in time, people, and resources necessary to work through and optimize the imaging approaches necessary to accomplish the projects proposed by the Center. Additionally, the Core will insure that the imaging approach used by each laboratory is uniformly agreed upon by each of the members of the Center and is of the highest possible quality.