Imaging Core

成像核心

• 批准号: 7625008
• 负责人: Joshua Trachtenberg
• 金额: $ 23.01万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-19 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7625008
• 关键词: Algorithms; Anatomy; Anterior; Cataloging; Catalogs; Computer software; Development; Genes; Image; Image Analysis; Interneurons; Kinetics; Label; Laboratories; Length; Life; Neurons; Operative Surgical Procedures; Preparation; Protocols documentation; Research Personnel; Resources; Role; Techniques; Testing; Time; Transgenic Mice; Vertebral column; Visual; Work; calcium indicator; cingulate cortex; cost; cost effective; density; hippocampal pyramidal neuron; in vivo; member; multidisciplinary; novel; relating to nervous system; sensor; tool; voltage

The function of the Imaging Core is to test novel imaging approaches and image analysis algorithms, streamline these techniques for in vivo imaging of cortical plasticity, and supply the optimized protocols and software to the investigators in the Center. The approaches optimized by the Imaging Core will be used in Projects 1, 2 and 3 of the Center for multidisciplinary, comprehensive studies of the role of specific genes in anatomical cortical plasticity. The Core is a cost effective and efficient way of providing imaging and analysis techniques to investigators in the Center. Specifically, the Core will: 1. Screen novel transgenic mice for markers for in vivo imaging that label specific aspects of neural anatomy, such as specific inhibitory interneurons and laminar-specific pyramidal neurons. 2. Catalog which labeled lines are bright enough for in vivo imaging and how this brightness changes with development. 3. Catalog which labeled lines express in visual, barrel, and anterior cingulate cortices 4. Screen newly-advanced genetically-encoded markers of neuronal function (e.g. calcium indicators and voltage sensors) to determine which are suitable for in vivo imaging. 5. Develop novel image analysis tools to automate the quantification of axonal and dendritic structural plasticity (e.g. changes in branching, branch length, spine volume, spine and bouton density, spine kinetics). 6. Continually fine-tune the surgical preparation for live imaging of cortical neurons. By assigning these tasks to one facility, the Center gains an economy of scale in which the time devoted to working through these important issues is absorbed once by the Core. Thus, each lab in the Center is not individually burdened with the costs in time, people, and resources necessary to work through and optimize the imaging approaches necessary to accomplish the projects proposed by the Center. Additionally, the Core will insure that the imaging approach used by each laboratory is uniformly agreed upon by each of the members of the Center and is of the highest possible quality.

成像核心的功能是测试新的成像方法和图像分析算法， 简化这些技术，用于皮质可塑性的体内成像，并提供优化的方案， 软件在中心的研究人员。成像核心优化的方法将用于 项目1，2和3中心的多学科，全面研究的作用，特定的基因， 解剖皮质可塑性 核心是一种具有成本效益和效率的方式，为研究人员提供成像和分析技术， 中心。具体而言，核心将： 1.筛选新的转基因小鼠的标记物，用于标记神经元特异性方面的体内成像。 解剖学，如特定的抑制性中间神经元和椎板特异性锥体神经元。 2.目录哪些标记线足够亮以进行体内成像，以及这种亮度如何随 发展 3.标记线在视觉、桶状和前扣带皮层中表达的目录 4.筛选神经元功能的新的先进遗传编码标志物（例如钙指标和 电压传感器）以确定哪些适合于体内成像。 5.开发新的图像分析工具，以自动量化轴突和树突结构 可塑性（例如分支、分支长度、棘体积、棘和终末密度、棘动力学的变化）。 6.不断微调皮层神经元实时成像的手术准备。 通过将这些任务分配给一个设施，中心获得了规模经济， 解决这些重要问题的工作被核心吸收一次。因此，中心的每个实验室都不是 每个人都承担着完成和优化工作所需的时间、人力和资源成本 完成中心提出的项目所需的成像方法。另夕h 核心将确保每个实验室使用的成像方法得到 该中心的成员，并尽可能高的质量。