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Control of Cell Systems Using Optically-Driven Nanoparticles as Actuators

使用光驱动纳米颗粒作为致动器控制细胞系统

基本信息

批准号：
7342420
负责人：
David Frank Russell
金额：
$ 18.01万
依托单位：
OHIO UNIVERSITY ATHENS
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-02-01 至 2009-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7342420
关键词：
Antibodies Apical Axon Binding Biochemical Biological Biological Assay Biomedical Technology Cauterization - action Cell surface Cells Clinical Research Collaborations Computers Coupled Data Deep Brain Stimulation Electronics Endocytosis Epithelium Extracellular Domain Face Figs - dietary Free Radicals Frequencies Germany Goals Gold Gold Colloid Heating Histocompatibility Human Human body Ice In Vitro Ion Channel Protein Label Lasers Lead Life Light Localized Measures Mediating Medical Medicine Modeling Molecular Probes Nervous system structure Neurons Neurotransmitter Receptor Operative Surgical Procedures Output Phase Physiological Processes Polymers Property Proteins Range Regulation Research Sensory Signal Transduction Site Stimulus Streptavidin Symptoms System Temperature Testing Tetanus Toxin Thermogenesis Time Tissues Toxic effect Transducers Tremor Visible Radiation Work absorption base cell type improved melting nanoparticle nanoshell nervous system disorder new technology novel relating to nervous system research study visual control

项目摘要

DESCRIPTION (provided by applicant): The PI and 3 co-PI's aim to develop new biomedical technology by validating the general principle that externally stimulated nanoparticles can be used as real-time actuators (input transducers) to exert control, perturbation, or regulation of biological systems by external stimuli, e.g. modulated light. Our approach is non-destructive and reversible, intended for information transfer to cells, over long times. Specifically, we aim to provide an experimental demonstration of this general principle, by altering the activity of a well-defined model nervous system using heat produced by light-stimulated gold nanoparticles, thereby causing a spatially localized and fast change in tissue temperature. We will apply gold nanoparticles to electroreceptors of paddlefish, as an experimentally advantageous and well-characterized test system. Our assay for temperature effects will be the frequencies of oscillators contained in each electroreceptor, whose fundamental frequencies (in the range of 20-70 Hz) vary with temperature at 1.8-3.4 Hz/¿C, as we measured previously. Several modes of applying gold nanoparticles will be evaluated to achieve spatially precise heating of different tissue components, including ejection from micropipets, endocytosis, or selective binding to cell surface molecules. Localization will be confirmed by co-delivery of fluorescent labels. We predict, and will evaluate, little toxicity from the gold nanoparticles. We will also test experimentally whether heating from gold nanoparticles can be used to reset the phase synchronization of neural oscillator networks. Quantitative analysis and modeling of heat flows will be used to reconcile our experimental data with the thermal properties of gold nanoparticles and live tissue. RELEVANCE: The proposed research may lead to medical applications, e.g. as a novel means of stimulating or regulating cell systems. This approach may be applicable to a wide range of cell types in the human body, because it is based on purely physical effects.

描述（由申请人提供）： PI 和 3 名共同 PI 的目标是通过验证外部刺激的纳米粒子可以用作实时执行器（输入传感器）以通过外部刺激（例如通过外部刺激）对生物系统进行控制、扰动或调节的一般原理来开发新的生物医学技术。调制光。我们的方法是非破坏性且可逆的，旨在将信息长时间传输到细胞。具体来说，我们的目标是通过利用光刺激金纳米颗粒产生的热量改变明确的模型神经系统的活动来提供这一一般原理的实验演示，从而引起组织温度的空间局部和快速变化。我们将把金纳米粒子应用到白鲟的电感受器上，作为一种实验上有利且特征良好的测试系统。我们对温度影响的分析将是每个电感受器中包含的振荡器的频率，正如我们之前测量的那样，其基频（在 20-70 Hz 范围内）随温度变化为 1.8-3.4 Hz/°C。将评估几种应用金纳米粒子的模式，以实现不同组织成分的空间精确加热，包括从微量移液器中喷射、内吞作用或选择性结合到细胞表面分子。定位将通过荧光标记的共同传递来确认。我们预测并将评估金纳米粒子的毒性很小。我们还将通过实验测试金纳米粒子的加热是否可用于重置神经振荡器网络的相位同步。热流的定量分析和建模将用于使我们的实验数据与金纳米粒子和活组织的热特性相一致。相关性：拟议的研究可能会带来医学应用，例如作为刺激或调节细胞系统的新方法。这种方法可能适用于人体内的多种细胞类型，因为它是基于纯粹的物理效应。