Molecular crosstalk between apical-basal polarity and planar cell polarity in axon guidance
轴突引导中顶端-基底极性和平面细胞极性之间的分子串扰
基本信息
- 批准号:401987-2013
- 负责人:
- 金额:$ 2.19万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Discovery Grants Program - Individual
- 财政年份:2017
- 资助国家:加拿大
- 起止时间:2017-01-01 至 2018-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The neurons or nerve cells have slender projections called axons that typically conduct electrical impulses away from the neuron's cell body. These axons need to locate and recognize their appropriate partners for correct wiring of the nervous system. To help them find their way in the developing embryo, axons are tipped with a highly motile and sensitive structure called the growth cone. While a large group of molecules that guide these growth cones have been identified, the signaling molecular pathways mediating this process remain largely unknown. Recent studies have implicated an important role for a pathway called planar cell polarity (PCP) pathway in axon guidance. PCP is the process by which cells become polarized in the plane of a tissue and is important for many developmental processes including neurulation. All epithelial cells exhibit another form of polarity called apical-basal (A-B) polarity that is important for their fundamental function. One of the protein complexes that regulate A-B polarity is called the Scrib complex and is composed of 3 proteins, Scribble (Scrib), Discs Large (Dlg) and Lethal giant larvae (Lgl). Recent studies have demonstrated molecular interactions between PCP and A-B polarity pathway suggesting common functions during embryonic development. We propose to investigate if the Scrib Complex provides an essential molecular link between the PCP pathway and A-B polarity in axon guidance in the developing spinal cord and brain. We will first study the expression pattern of each of Scrib, Dlg and Lgl in the developing brain and spinal cord and compare these patterns to those of 3 PCP genes called Vangl2, Frizzled3 and Celsr3. Next, we will knockdown the expression of each of Scrib, Dlg and Lgl in spinal cord preparations and brain cultures from wild-type and from mice mutant for the PCP gene Vangl2 and we will use various labeling approaches to investigate any resulting defect in axon guidance. Our study will provide important insights into the signaling pathways involved in axon guidance that are essential for proper wiring of the central nervous system.
神经元或神经细胞有细长的突起,称为轴突,通常将电脉冲从神经元的细胞体传导出去。这些轴突需要定位和识别它们合适的伙伴,以正确连接神经系统。为了帮助它们在发育中的胚胎中找到方向,轴突顶端有一个高度能动和敏感的结构,称为生长锥。虽然已经鉴定了一大组引导这些生长锥的分子,但介导这一过程的信号分子途径在很大程度上仍然未知。最近的研究表明,一条称为平面细胞极性(PCP)的通路在轴突导向中起着重要作用。PCP是细胞在组织平面中极化的过程,对包括神经形成在内的许多发育过程都很重要。所有上皮细胞都表现出另一种形式的极性,称为顶基(A-B)极性,这对它们的基本功能很重要。调节A-B极性的蛋白质复合物之一被称为Scrib复合物,由3种蛋白质组成,Scribble(Scrib),Discs Large(Dlg)和Lethal giant larvae(Lgl)。最近的研究表明,PCP和A-B极性通路之间的分子相互作用表明在胚胎发育过程中具有共同的功能。我们建议调查,如果Scrib复合物提供了一个重要的分子之间的联系PCP途径和A-B极性的轴突指导在发展中的脊髓和大脑。我们将首先研究Scrib,Dlg和Lgl在发育中的大脑和脊髓中的表达模式,并将这些模式与称为Vangl 2,Frizzled 3和Celsr 3的3种PCP基因的表达模式进行比较。接下来,我们将敲低来自野生型和来自PCP基因Vangl 2的小鼠突变体的脊髓制备物和脑培养物中的Scrib、Dlg和Lgl中的每一种的表达,并且我们将使用各种标记方法来研究轴突引导中的任何导致的缺陷。我们的研究将提供重要的见解,参与轴突指导的信号通路是必不可少的适当布线的中枢神经系统。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Kibar, Zoha其他文献
Loss-of-function de novo mutations play an important role in severe human neural tube defects
- DOI:
10.1136/jmedgenet-2015-103027 - 发表时间:
2015-07-01 - 期刊:
- 影响因子:4
- 作者:
Lemay, Philippe;Guyot, Marie-Claude;Kibar, Zoha - 通讯作者:
Kibar, Zoha
Mutations in VANGL1 associated with neural-tube defects
- DOI:
10.1056/nejmoa060651 - 发表时间:
2007-04-05 - 期刊:
- 影响因子:158.5
- 作者:
Kibar, Zoha;Torban, Elena;Gros, Philippe - 通讯作者:
Gros, Philippe
Identification and characterization of novel rare mutations in the planar cell polarity gene PRICKLE1 in human neural tube defects.
- DOI:
10.1002/humu.21589 - 发表时间:
2011-12 - 期刊:
- 影响因子:3.9
- 作者:
Bosoi, Ciprian M.;Capra, Valeria;Allache, Redouane;Vincent Quoc-Huy Trinh;De Marco, Patrizia;Merello, Elisa;Drapeau, Pierre;Bassuk, Alexander G.;Kibar, Zoha - 通讯作者:
Kibar, Zoha
Rare deleterious variants in GRHL3 are associated with human spina bifida
- DOI:
10.1002/humu.23214 - 发表时间:
2017-06-01 - 期刊:
- 影响因子:3.9
- 作者:
Lemay, Philippe;De Marco, Patrizia;Kibar, Zoha - 通讯作者:
Kibar, Zoha
Novel mutations in VANGL1 in neural tube defects.
- DOI:
10.1002/humu.21026 - 发表时间:
2009-07 - 期刊:
- 影响因子:3.9
- 作者:
Kibar, Zoha;Bosoi, Ciprian M.;Kooistra, Megan;Salem, Sandra;Finnell, Richard H.;De Marco, Patrizia;Merello, Elisa;Bassuk, Alexander G.;Capra, Valeria;Gros, Philippe - 通讯作者:
Gros, Philippe
Kibar, Zoha的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Kibar, Zoha', 18)}}的其他基金
Molecular crosstalk between apical-basal polarity and planar cell polarity in axon guidance
轴突引导中顶端-基底极性和平面细胞极性之间的分子串扰
- 批准号:
RGPIN-2018-06073 - 财政年份:2022
- 资助金额:
$ 2.19万 - 项目类别:
Discovery Grants Program - Individual
Molecular crosstalk between apical-basal polarity and planar cell polarity in axon guidance
轴突引导中顶端-基底极性和平面细胞极性之间的分子串扰
- 批准号:
RGPIN-2018-06073 - 财政年份:2021
- 资助金额:
$ 2.19万 - 项目类别:
Discovery Grants Program - Individual
Molecular crosstalk between apical-basal polarity and planar cell polarity in axon guidance
轴突引导中顶端-基底极性和平面细胞极性之间的分子串扰
- 批准号:
RGPIN-2018-06073 - 财政年份:2020
- 资助金额:
$ 2.19万 - 项目类别:
Discovery Grants Program - Individual
Molecular crosstalk between apical-basal polarity and planar cell polarity in axon guidance
轴突引导中顶端-基底极性和平面细胞极性之间的分子串扰
- 批准号:
RGPIN-2018-06073 - 财政年份:2019
- 资助金额:
$ 2.19万 - 项目类别:
Discovery Grants Program - Individual
Molecular crosstalk between apical-basal polarity and planar cell polarity in axon guidance
轴突引导中顶端-基底极性和平面细胞极性之间的分子串扰
- 批准号:
RGPIN-2018-06073 - 财政年份:2018
- 资助金额:
$ 2.19万 - 项目类别:
Discovery Grants Program - Individual
Molecular crosstalk between apical-basal polarity and planar cell polarity in axon guidance
轴突引导中顶端-基底极性和平面细胞极性之间的分子串扰
- 批准号:
401987-2013 - 财政年份:2016
- 资助金额:
$ 2.19万 - 项目类别:
Discovery Grants Program - Individual
Molecular crosstalk between apical-basal polarity and planar cell polarity in axon guidance
轴突引导中顶端-基底极性和平面细胞极性之间的分子串扰
- 批准号:
401987-2013 - 财政年份:2015
- 资助金额:
$ 2.19万 - 项目类别:
Discovery Grants Program - Individual
Molecular crosstalk between apical-basal polarity and planar cell polarity in axon guidance
轴突引导中顶端-基底极性和平面细胞极性之间的分子串扰
- 批准号:
401987-2013 - 财政年份:2014
- 资助金额:
$ 2.19万 - 项目类别:
Discovery Grants Program - Individual
Molecular crosstalk between apical-basal polarity and planar cell polarity in axon guidance
轴突引导中顶端-基底极性和平面细胞极性之间的分子串扰
- 批准号:
401987-2013 - 财政年份:2013
- 资助金额:
$ 2.19万 - 项目类别:
Discovery Grants Program - Individual
相似国自然基金
基于TLR4/NF-κB与PPAR-γ/CD36信号通路间的crosstalk探讨黄芪多糖促进脑出血后血肿吸收的机制
- 批准号:JCZRQN202500623
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
西黄胶囊通过NLRP3/Caspase1/GSDMD/Beclin-1信号通路调控细胞焦亡-自噬Crosstalk机制治疗GLM的应用基础研究
- 批准号:2025JJ80699
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
Spp1介导肾-肌crosstalk参与儿童CKD致肌少症的作用和机制
研究
- 批准号:
- 批准年份:2024
- 资助金额:0.0 万元
- 项目类别:省市级项目
基于外泌体源性线粒体介导小胶质细胞-神经元crosstalk探讨改善AD认知的机制研究
- 批准号:
- 批准年份:2024
- 资助金额:0.0 万元
- 项目类别:省市级项目
基于线粒体生物发生介导的软骨细胞-巨噬细胞衰老Crosstalk探讨曲直汤延缓KOA进展的作用机制
- 批准号:
- 批准年份:2024
- 资助金额:0.0 万元
- 项目类别:省市级项目
基于胰岛巨噬细胞-β细胞免疫Crosstalk诱导β细胞去分化研究T2DM“火”-“气”互作科学内涵及泻火固正法干预机制
- 批准号:
- 批准年份:2024
- 资助金额:15.0 万元
- 项目类别:省市级项目
HDACs信号通路通过与星形胶质细胞NF-κb信号通路Crosstalk调控TREM2+小胶质细胞影响糖尿病脑卒中后神经功能的研究
- 批准号:
- 批准年份:2024
- 资助金额:15.0 万元
- 项目类别:省市级项目
基于Exos-miRHAs探讨大量灵仙方调控肝巨细胞-胆管细胞crosstalk参与胆管炎性-EMT持抗胆石形成的机制研究
- 批准号:
- 批准年份:2024
- 资助金额:0 万元
- 项目类别:面上项目
“肌-脑crosstalk”视阈下太极拳训练对2型糖尿病合并脑小血管病患者的脑血管保护效应研究
- 批准号:2024Y9478
- 批准年份:2024
- 资助金额:50.0 万元
- 项目类别:省市级项目
复方肠泰方通过“ 精氨酸甲基化-泛素化crosstalk”调控铁死亡
的抗结直肠癌作用机制研究
- 批准号:
- 批准年份:2024
- 资助金额:0.0 万元
- 项目类别:省市级项目
相似海外基金
Dissecting the molecular crosstalk between mechanotransduction and primary cilia in models of congenital valvulopathies
剖析先天性瓣膜病模型中机械转导和初级纤毛之间的分子串扰
- 批准号:
MR/X019837/1 - 财政年份:2023
- 资助金额:
$ 2.19万 - 项目类别:
Research Grant
Molecular crosstalk between apical-basal polarity and planar cell polarity in axon guidance
轴突引导中顶端-基底极性和平面细胞极性之间的分子串扰
- 批准号:
RGPIN-2018-06073 - 财政年份:2022
- 资助金额:
$ 2.19万 - 项目类别:
Discovery Grants Program - Individual
Molecular crosstalk between pollutant and hypoxia exposure in islets
污染物与胰岛缺氧暴露之间的分子串扰
- 批准号:
573489-2022 - 财政年份:2022
- 资助金额:
$ 2.19万 - 项目类别:
University Undergraduate Student Research Awards
Elucidation of the molecular basis of crosstalk between metabolism and inflammation in vascular endothelial cell activation
阐明血管内皮细胞活化中代谢与炎症之间串扰的分子基础
- 批准号:
22H02891 - 财政年份:2022
- 资助金额:
$ 2.19万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
The molecular mechanism of the crosstalk between the beta-2 adrenergic receptor and chemokine receptors in lymphocytes
淋巴细胞β2肾上腺素受体与趋化因子受体串扰的分子机制
- 批准号:
22K07118 - 财政年份:2022
- 资助金额:
$ 2.19万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Establishment of the molecular basis for the treatment of endometriosis based on the crosstalk between hepcidin and PGC1a
基于hepcidin与PGC1a串扰建立子宫内膜异位症治疗的分子基础
- 批准号:
21K16775 - 财政年份:2021
- 资助金额:
$ 2.19万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Molecular crosstalk between apical-basal polarity and planar cell polarity in axon guidance
轴突引导中顶端-基底极性和平面细胞极性之间的分子串扰
- 批准号:
RGPIN-2018-06073 - 财政年份:2021
- 资助金额:
$ 2.19万 - 项目类别:
Discovery Grants Program - Individual
Elucidation of the molecular mechanisms of crosstalk between inflammatory cells with somatic mutations and cancer cells
阐明体细胞突变炎症细胞与癌细胞之间串扰的分子机制
- 批准号:
20K21535 - 财政年份:2020
- 资助金额:
$ 2.19万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)
Molecular crosstalk between apical-basal polarity and planar cell polarity in axon guidance
轴突引导中顶端-基底极性和平面细胞极性之间的分子串扰
- 批准号:
RGPIN-2018-06073 - 财政年份:2020
- 资助金额:
$ 2.19万 - 项目类别:
Discovery Grants Program - Individual
Molecular crosstalk between apical-basal polarity and planar cell polarity in axon guidance
轴突引导中顶端-基底极性和平面细胞极性之间的分子串扰
- 批准号:
RGPIN-2018-06073 - 财政年份:2019
- 资助金额:
$ 2.19万 - 项目类别:
Discovery Grants Program - Individual