Identification of Immune Targets in Psoriatic Arthritis

进度报告 P&F 6 -银屑病关节炎免疫靶点的鉴定 PI：Mark Soloski博士 我们提出了一系列旨在鉴定免疫靶向候选分子种类的研究。 银屑病关节炎的治疗方法在这项研究中，我们建议：1。银屑病关节炎（PsA）血清筛查 患者和正常对照中是否存在对细胞蛋白质类具有反应性的抗体。2. 使用免疫组织化学方法鉴定和表征PsA中自身抗体识别的靶向分子种类。 蛋白质组学方法。 在本研究的初始阶段，我们收集并存档了血清、PMBC以及受影响和未受影响的 43例正常人和23例PsA患者的皮肤样本。此外，我们还建立了一种蛋白质印迹法， 我们将采用这种方法来帮助识别PsA自身免疫识别的蛋白质种类 血清的到目前为止，我们已经成功地测试了角质细胞细胞提取物与这种蛋白质印迹法 对于SLE中靶向自身抗原（U160 k和PARP）的存在，发现该方法敏感， 可靠我们目前正在为一系列细胞类型生成细胞提取物，这些细胞类型将用于本研究。 approach.

Progress Report P&F 6 - Identification of Immune Targets in Psoriatic Arthritis PI: Mark Soloski, Ph.D. We propose a series of studies aimed at identifying candidate molecular species targeted for immune recognition in Psoriatic Arthritis. In this study, we propose to: 1. Screen serum from Psoriatic Arthritis (PsA) patients and normal controls for the presence of antibodies reactive toward cellular protein species. 2. Identify and characterize the molecular species targeted for recognition by autoantiboclies in PsA using a proteomics based approach. In the initial phases of this study we have collected and archived serum, PMBCs and affected and unaffected skin samples from 43 normal and 23 PsA patients. In addition we have established a western blotting approach that we will employ to help identify proteins species targeted for recognition by PsA autoimmune serum. To date we have successfully tested keratinocyte cellular extracts with this western blotting approach for the presence of autoantigens targeted in SLE (U160k and PARP) and found the approach sensitive and reliable. We are currently generating cell extracts for a range of cell types which will be utilized in this approach.