Identification of Immune Targets in Psoriatic Arthritis

进度报告 P&F 6-银屑病关节炎免疫靶点的鉴定 派：马克·索洛斯基，博士。 我们提出了一系列旨在识别免疫目标候选分子物种的研究 对银屑病关节炎的认识。在本研究中，我们建议：1.筛选银屑病关节炎(PSA)患者血清 患者和正常对照存在针对细胞蛋白种类的抗体反应。2. 鉴定和表征PSA中自身抗体识别的目标分子物种 基于蛋白质组学的方法。 在这项研究的初始阶段，我们收集并存档了血清、PMBC以及受影响和未受影响的 43例正常人和23例PSA患者的皮肤标本。此外，我们还建立了一个Western blotting 我们将用来帮助识别PSA自身免疫识别目标的蛋白质种类的方法 血清。到目前为止，我们已经成功地用这种蛋白质印迹方法测试了角质形成细胞的提取液。 针对SLE中靶向自身抗原的存在(U160k和PARP)，发现该方法敏感和 可靠。我们目前正在为一系列细胞类型生成细胞提取液，这些提取液将用于 接近。

Progress Report P&F 6 - Identification of Immune Targets in Psoriatic Arthritis PI: Mark Soloski, Ph.D. We propose a series of studies aimed at identifying candidate molecular species targeted for immune recognition in Psoriatic Arthritis. In this study, we propose to: 1. Screen serum from Psoriatic Arthritis (PsA) patients and normal controls for the presence of antibodies reactive toward cellular protein species. 2. Identify and characterize the molecular species targeted for recognition by autoantiboclies in PsA using a proteomics based approach. In the initial phases of this study we have collected and archived serum, PMBCs and affected and unaffected skin samples from 43 normal and 23 PsA patients. In addition we have established a western blotting approach that we will employ to help identify proteins species targeted for recognition by PsA autoimmune serum. To date we have successfully tested keratinocyte cellular extracts with this western blotting approach for the presence of autoantigens targeted in SLE (U160k and PARP) and found the approach sensitive and reliable. We are currently generating cell extracts for a range of cell types which will be utilized in this approach.