Identification of Immune Targets in Psoriatic Arthritis

银屑病关节炎免疫靶点的鉴定

• 批准号: 7674130
• 负责人: Mark J Soloski
• 金额: $ 1.16万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7674130
• 关键词: Affect; Antibodies; Archives; Autoantigens; Cell Extracts; Doctor of Philosophy; Immune Targeting; Molecular; Patients; Phase; Progress Reports; Proteins; Proteomics; Psoriatic Arthritis; Range; Sampling; Series; Serum; Skin; Systemic Lupus Erythematosus; Testing; Western Blotting; autoimmune arthritis; base; cell type; keratinocyte

Progress Report P&F 6 - Identification of Immune Targets in Psoriatic Arthritis PI: Mark Soloski, Ph.D. We propose a series of studies aimed at identifying candidate molecular species targeted for immune recognition in Psoriatic Arthritis. In this study, we propose to: 1. Screen serum from Psoriatic Arthritis (PsA) patients and normal controls for the presence of antibodies reactive toward cellular protein species. 2. Identify and characterize the molecular species targeted for recognition by autoantiboclies in PsA using a proteomics based approach. In the initial phases of this study we have collected and archived serum, PMBCs and affected and unaffected skin samples from 43 normal and 23 PsA patients. In addition we have established a western blotting approach that we will employ to help identify proteins species targeted for recognition by PsA autoimmune serum. To date we have successfully tested keratinocyte cellular extracts with this western blotting approach for the presence of autoantigens targeted in SLE (U160k and PARP) and found the approach sensitive and reliable. We are currently generating cell extracts for a range of cell types which will be utilized in this approach.

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