Flow Cytometry Core Center BD FACSAria

描述（由申请人提供）：本申请申请资金用于购买细胞分选器，该分选器将成为位于约翰霍普金斯医学院湾景医学院的流式细胞仪核心设备的一部分。这一要求是由研究人员核心小组对细胞分选能力和多参数流式细胞术的科学需求驱动的。目前，在这个校园站点上还没有细胞分选，获得这种仪器将对当前和未来的研究项目产生重大影响。在该提案中，位于湾景校区的11个主要用户和3个次要用户描述了他们的研究活动，以及他们将如何从所要求的细胞分类器中受益。研究领域包括：变应性疾病机制、肥大细胞免疫生物学、衰老中的免疫机制、自身免疫的诱导与调控、感染中的粘膜与先天免疫机制、肺移植的免疫病理。我们建议购买的仪器是Becton-Dickenson公司生产的FACSAria细胞分选仪。该仪器将配备四个固态激光器，一个488nm氩激光器，一个638氦激光器，一个407紫外激光器和一个355紫外激光器。此外，该仪器还将配备一个自动细胞沉积装置和一个气溶胶密封装置。选择仪器、激光器和附件，以满足用户群体的多参数和细胞分选需求。该仪器将成为约翰霍普金斯大学湾景校区最近建立的流式细胞术核心设施的一部分，并将满足该研究界的流式细胞术需求。该设施将配备一名全职经理/操作员，并由一名设施主任和一个咨询委员会监督。这一设施的设立将通过最初三年的机构种子投资得到支助，其中包括用于工作人员、用品和服务合同的资源。政府还制定了一项长期财政计划，使该基金能够自我维持。该设施的建立和细胞分选能力的获得将共同加强与公共卫生有关的一些既定生物医学研究领域，包括哮喘和过敏、自身免疫、肺功能、衰老和传染病。

DESCRIPTION (provided by applicant): This application requests funds for the purchase of a cell sorter that will be part of a flow cytometry core facility located on the Bayview Medical Campus of the Johns Hopkins School of Medicine. This request is driven by the scientific demand for cell sorting capability and multi-parameter flow cytometry by a core group of investigators. At the present time cell sorting is not available on this campus site and the acquisition of this instrumentation will have a major impact on current and future research programs. Within this proposal, 11 major and 3 minor users located on the Bayview Campus describe their research activities and how these will benefit from the requested cell sorter. The research areas include: mechanisms of allergic disease, mast cell immunobiology, immune mechanisms in aging, induction and regulation of autoimmunity, mucosal and innate immune mechanisms in infection and immunopathology of lung transplantation. The instrument we are proposing to purchase is a FACSAria cell sorter manufactured by Becton-Dickenson. This instrument will be equipped with four solid-state lasers, a 488nm argon, 638 HeNe, 407 UV and a 355UV. In addition the instrument will be equipped with an automatic cell deposition unit and an aerosol containment device. The instrument, lasers and accessories were chosen to meet the multiparameter and cell sorting needs of the user group. The instrument will be part of a recently established flow cytometry core facility on the Johns Hopkins Bayview Campus and will meet the flow cytometry needs of that research community. The facility will be staffed by a full time manager/operator and will be overseen by a facility director and an advisory committee. The establishment of this facility will be supported through institutional seed investment for the initial three years and includes resources for staffing, supplies and service contracts. A long-range fiscal plan is also planned that will allow the facility to be self-sustaining. Collectively the establishment of this facility and the acquisition of cell sorting capability will enhance a number of established biomedical research areas relevant to public health including asthma and allergy, autoimmunity, pulmonary function, aging and infectious diseases.